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Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer

Breast cancer incidence rate has increased in the 5 recent years with 14% increases in mortality. The structural change in the collagen chain has led to alterations in the cancer cells. Various biological processes, such as differentiation or gene expression, are regulated through extracelullar matr...

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Main Authors: Mat Yusof , Afzan, Mohammad , Mardhiah, Syed Bahrom, Sharifah Norbaizura, Mohideen, Syahirah Kaja, Roshdi, Ridhwan, Parman, Adibah
Format: Article
Language:English
Published: Penerbit UTM Press 2015
Subjects:
Q Science (General)
RA0421 Public health. Hygiene. Preventive Medicine
Online Access:http://irep.iium.edu.my/46359/1/6752-18539-1-SM_%281%29AFZAN.pdf
http://irep.iium.edu.my/46359/
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spelling my.iium.irep.46359 http://irep.iium.edu.my/46359/ Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer Mat Yusof , Afzan Mohammad , Mardhiah Syed Bahrom, Sharifah Norbaizura Mohideen, Syahirah Kaja Roshdi, Ridhwan Parman, Adibah Q Science (General) RA0421 Public health. Hygiene. Preventive Medicine Breast cancer incidence rate has increased in the 5 recent years with 14% increases in mortality. The structural change in the collagen chain has led to alterations in the cancer cells. Various biological processes, such as differentiation or gene expression, are regulated through extracelullar matrix (ECM)[1]. The restructuring of the collagenous architecture in the hypoxic microenvironment may influence the invasive growth of the cancer cells. With the increased stress within the cell, the invasion of cancer cells into the ECM was triggered. This cell lines model would enable the exploration of the relationship between the extracellular matrices component and the tumor proliferation. The aim of this study is to characterize the collagen (IV) mRNA expression in the breast cancer cell. Breast cancer (MCF7) cell lines were cultured and harvested upon confluent. The RNA was extracted from the cell lines and then the cDNA were synthesized. The collagen (IV) mRNA levels in breast cancer cell lines were measured using real time PCR and GAPDH was used as an internal control. The level of COL4A2 (IV) mRNA expression was higher compared with COL4A1 (IV) mRNA. The level of COL4A5 (IV) mRNA was reduced significantly in breast cancer cells lines. Overall, the expression of COL4A1-A6 (IV) was reduced. The reduced amount of collagen (IV) in breast cancer cell lines suggested that the collagen was restructured and this has triggered the tumor invasion into the ECM. Penerbit UTM Press 2015-10-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46359/1/6752-18539-1-SM_%281%29AFZAN.pdf Mat Yusof , Afzan and Mohammad , Mardhiah and Syed Bahrom, Sharifah Norbaizura and Mohideen, Syahirah Kaja and Roshdi, Ridhwan and Parman, Adibah (2015) Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer. Jurnal Teknologi, 77 (25). pp. 133-136. ISSN eISSN 2180-3722 DOI: http://dx.doi.org/10.11113/jt.v77.6752
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle Q Science (General)
RA0421 Public health. Hygiene. Preventive Medicine
Mat Yusof , Afzan
Mohammad , Mardhiah
Syed Bahrom, Sharifah Norbaizura
Mohideen, Syahirah Kaja
Roshdi, Ridhwan
Parman, Adibah
Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
description Breast cancer incidence rate has increased in the 5 recent years with 14% increases in mortality. The structural change in the collagen chain has led to alterations in the cancer cells. Various biological processes, such as differentiation or gene expression, are regulated through extracelullar matrix (ECM)[1]. The restructuring of the collagenous architecture in the hypoxic microenvironment may influence the invasive growth of the cancer cells. With the increased stress within the cell, the invasion of cancer cells into the ECM was triggered. This cell lines model would enable the exploration of the relationship between the extracellular matrices component and the tumor proliferation. The aim of this study is to characterize the collagen (IV) mRNA expression in the breast cancer cell. Breast cancer (MCF7) cell lines were cultured and harvested upon confluent. The RNA was extracted from the cell lines and then the cDNA were synthesized. The collagen (IV) mRNA levels in breast cancer cell lines were measured using real time PCR and GAPDH was used as an internal control. The level of COL4A2 (IV) mRNA expression was higher compared with COL4A1 (IV) mRNA. The level of COL4A5 (IV) mRNA was reduced significantly in breast cancer cells lines. Overall, the expression of COL4A1-A6 (IV) was reduced. The reduced amount of collagen (IV) in breast cancer cell lines suggested that the collagen was restructured and this has triggered the tumor invasion into the ECM.
format Article
author Mat Yusof , Afzan
Mohammad , Mardhiah
Syed Bahrom, Sharifah Norbaizura
Mohideen, Syahirah Kaja
Roshdi, Ridhwan
Parman, Adibah
author_facet Mat Yusof , Afzan
Mohammad , Mardhiah
Syed Bahrom, Sharifah Norbaizura
Mohideen, Syahirah Kaja
Roshdi, Ridhwan
Parman, Adibah
author_sort Mat Yusof , Afzan
title Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
title_short Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
title_full Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
title_fullStr Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
title_full_unstemmed Characterization of Collagen (IV) mRNA in Cell Lines of Breast Cancer
title_sort characterization of collagen (iv) mrna in cell lines of breast cancer
publisher Penerbit UTM Press
publishDate 2015
url http://irep.iium.edu.my/46359/1/6752-18539-1-SM_%281%29AFZAN.pdf
http://irep.iium.edu.my/46359/
_version_ 1643616869614092288
score 13.211869

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