Encapsulated embryogenic callus of clitoria ternatea L. for regeneration and conservation
Encapsulated embryogenic callus of Clitoria ternatea L. were successfully created from leaf explants within 3 weeks after germination on Murashige and Skoog (MS) media. The seeds were initially washed with tap water and teepol, then the seeds were sterilised with 99% (v/v) sodium hypochlorite s...
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my.iium.irep.457722016-10-17T04:57:24Z http://irep.iium.edu.my/45772/ Encapsulated embryogenic callus of clitoria ternatea L. for regeneration and conservation Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Elias, Hashimah Saleh, Azani QK Botany SB Plant culture TP248.13 Biotechnology Encapsulated embryogenic callus of Clitoria ternatea L. were successfully created from leaf explants within 3 weeks after germination on Murashige and Skoog (MS) media. The seeds were initially washed with tap water and teepol, then the seeds were sterilised with 99% (v/v) sodium hypochlorite solution for 1 minute and rinsed with distilled water three times. In a laminar flow cabinet, the seeds were dipped in 70% (v/v) ethanol for 1 minute and blotted with steriled tissue. The 3 mm2 leaf explants were encapsulated with 3% alginate (w/v) which were suplemented with various concentrations (0.5-2.5 mg l-1 ) and combinations of NAA, BAP and adenine. The optimum concentration for the formation of encapsulation matrix was 3.0% sodium alginate (NaC6H7O6 ). Encapsulated beads were soaked in 100 mM calcium chloride dehydrate (CaCl2 .2H2O) solution for 30 minutes. No suitable beads were formed with low concentration (1-2%) of sodium alginate. Within 10 minutes soaking in calcium chloride dehydrate, clear and bead formation with no definite shape was observed. While, within 20 minutes in calcium chloride dehydrate, clear beads, solid and round in shape was observed, however, inside the bead was still in liquid condition. In the present study, the rate of germination of synthetic seeds were slightly decreased from 100% to 77% after 60 days of storage at 4°C. Embryogenic tissue from leaf explants of Clitoria ternatea was distinguished by double staining method with bright red of acetocarmine. This technology is an alternative and supplementary method for regeneration, mass propagation and conservation of this medicinal, attractive ornamental and also forage crop for future uses and exploitation IACSIT Press 2016-05 Article REM application/pdf en http://irep.iium.edu.my/45772/1/PAPER_2.pdf Mahmad, Noraini and Mat Taha, Rosna and Othman, Rashidi and Elias, Hashimah and Saleh, Azani (2016) Encapsulated embryogenic callus of clitoria ternatea L. for regeneration and conservation. International Journal of Environmental Science and Development (IJESD), 7 (5). pp. 363-367. ISSN 2010-0264 http://www.ijesd.org/ 10.7763/IJESD.2016.V7.801 |
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QK Botany SB Plant culture TP248.13 Biotechnology |
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QK Botany SB Plant culture TP248.13 Biotechnology Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Elias, Hashimah Saleh, Azani Encapsulated embryogenic callus of clitoria ternatea L. for regeneration and conservation |
| description |
Encapsulated embryogenic callus of Clitoria
ternatea L. were successfully created from leaf explants within 3
weeks after germination on Murashige and Skoog (MS) media.
The seeds were initially washed with tap water and teepol, then
the seeds were sterilised with 99% (v/v) sodium hypochlorite
solution for 1 minute and rinsed with distilled water three times.
In a laminar flow cabinet, the seeds were dipped in 70% (v/v)
ethanol for 1 minute and blotted with steriled tissue. The 3 mm2
leaf explants were encapsulated with 3% alginate (w/v) which
were suplemented with various concentrations (0.5-2.5 mg l-1
)
and combinations of NAA, BAP and adenine. The optimum
concentration for the formation of encapsulation matrix was
3.0% sodium alginate (NaC6H7O6
). Encapsulated beads were
soaked in 100 mM calcium chloride dehydrate (CaCl2
.2H2O)
solution for 30 minutes. No suitable beads were formed with low
concentration (1-2%) of sodium alginate. Within 10 minutes
soaking in calcium chloride dehydrate, clear and bead formation
with no definite shape was observed. While, within 20 minutes in
calcium chloride dehydrate, clear beads, solid and round in
shape was observed, however, inside the bead was still in liquid
condition. In the present study, the rate of germination of
synthetic seeds were slightly decreased from 100% to 77% after
60 days of storage at 4°C. Embryogenic tissue from leaf explants
of Clitoria ternatea was distinguished by double staining method
with bright red of acetocarmine. This technology is an
alternative and supplementary method for regeneration, mass
propagation and conservation of this medicinal, attractive
ornamental and also forage crop for future uses and
exploitation |
| format |
Article |
| author |
Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Elias, Hashimah Saleh, Azani |
| author_facet |
Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Elias, Hashimah Saleh, Azani |
| author_sort |
Mahmad, Noraini |
| title |
Encapsulated embryogenic callus of clitoria ternatea L.
for regeneration and conservation |
| title_short |
Encapsulated embryogenic callus of clitoria ternatea L.
for regeneration and conservation |
| title_full |
Encapsulated embryogenic callus of clitoria ternatea L.
for regeneration and conservation |
| title_fullStr |
Encapsulated embryogenic callus of clitoria ternatea L.
for regeneration and conservation |
| title_full_unstemmed |
Encapsulated embryogenic callus of clitoria ternatea L.
for regeneration and conservation |
| title_sort |
encapsulated embryogenic callus of clitoria ternatea l.
for regeneration and conservation |
| publisher |
IACSIT Press |
| publishDate |
2016 |
| url |
http://irep.iium.edu.my/45772/1/PAPER_2.pdf http://irep.iium.edu.my/45772/ http://www.ijesd.org/ |
| _version_ |
1643612856565891072 |
| score |
13.211869 |