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Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae

Streptococcus pneumoniae is an important pathogen cause various severe illness in human particularly pneumonia, septicaemia and meningitis. One of its virulence factors is hyaluronate lyase, an enzyme which degrades hyaluronic acid (HA), a major component in extracellular matrix of human connective...

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Main Authors: Ahmad Yusof @ Hanafi, Hanani, Mohd Desa, Mohd Nasir, Masri, Siti Norbaya, Osman, Malina, Jamal, Farida
Format: Conference or Workshop Item
Language:English
English
Published: 2015
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/45523/8/Poster_ICBHSR_15_Hanani_4.pdf
http://irep.iium.edu.my/45523/10/Dr_Hanani_2015.pdf
http://irep.iium.edu.my/45523/
https://www.facebook.com/ICBHSR/info?tab=page_info
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spelling my.iium.irep.455232016-02-03T02:24:20Z http://irep.iium.edu.my/45523/ Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae Ahmad Yusof @ Hanafi, Hanani Mohd Desa, Mohd Nasir Masri, Siti Norbaya Osman, Malina Jamal, Farida QR Microbiology Streptococcus pneumoniae is an important pathogen cause various severe illness in human particularly pneumonia, septicaemia and meningitis. One of its virulence factors is hyaluronate lyase, an enzyme which degrades hyaluronic acid (HA), a major component in extracellular matrix of human connective tissues. Degradation of HA decrease host tissue viscosity and eventually increase tissue permeability to allow migration of pneumococcus or its product into human tissues. The study attempted to screen production of hyaluronate lyase in 100 isolates of S. pneumoniae from various sites and year of isolation, and other bacterial characteristics. Ninety-six isolates (96%) showed positive hyaluronate lyase through hyaluronic acid diffusion agar method. Few positive hyaluronate lyase isolates were selected and proceed to enzymatic activity determination through hyaluronic acid turbidity reduction assay. The study found that non-vaccine serotypes and carriage isolates showed high hyaluronate lyase activity than invasive isolates. This finding suggest that hyaluronate lyase in S. pneumoniae might play more important role in pneumococcal colonization than invasion. 2015 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/45523/8/Poster_ICBHSR_15_Hanani_4.pdf application/pdf en http://irep.iium.edu.my/45523/10/Dr_Hanani_2015.pdf Ahmad Yusof @ Hanafi, Hanani and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Osman, Malina and Jamal, Farida (2015) Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae. In: International Conference on Biomedical and Health Sciences Research 2015, 25-27 Jan 2015, The Everly Hotel Putrajaya. (Unpublished) https://www.facebook.com/ICBHSR/info?tab=page_info
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QR Microbiology
spellingShingle QR Microbiology
Ahmad Yusof @ Hanafi, Hanani
Mohd Desa, Mohd Nasir
Masri, Siti Norbaya
Osman, Malina
Jamal, Farida
Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
description Streptococcus pneumoniae is an important pathogen cause various severe illness in human particularly pneumonia, septicaemia and meningitis. One of its virulence factors is hyaluronate lyase, an enzyme which degrades hyaluronic acid (HA), a major component in extracellular matrix of human connective tissues. Degradation of HA decrease host tissue viscosity and eventually increase tissue permeability to allow migration of pneumococcus or its product into human tissues. The study attempted to screen production of hyaluronate lyase in 100 isolates of S. pneumoniae from various sites and year of isolation, and other bacterial characteristics. Ninety-six isolates (96%) showed positive hyaluronate lyase through hyaluronic acid diffusion agar method. Few positive hyaluronate lyase isolates were selected and proceed to enzymatic activity determination through hyaluronic acid turbidity reduction assay. The study found that non-vaccine serotypes and carriage isolates showed high hyaluronate lyase activity than invasive isolates. This finding suggest that hyaluronate lyase in S. pneumoniae might play more important role in pneumococcal colonization than invasion.
format Conference or Workshop Item
author Ahmad Yusof @ Hanafi, Hanani
Mohd Desa, Mohd Nasir
Masri, Siti Norbaya
Osman, Malina
Jamal, Farida
author_facet Ahmad Yusof @ Hanafi, Hanani
Mohd Desa, Mohd Nasir
Masri, Siti Norbaya
Osman, Malina
Jamal, Farida
author_sort Ahmad Yusof @ Hanafi, Hanani
title Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
title_short Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
title_full Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
title_fullStr Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
title_full_unstemmed Hyaluronate lyase activity in Malaysian Isolates of Streptococcus pneumoniae
title_sort hyaluronate lyase activity in malaysian isolates of streptococcus pneumoniae
publishDate 2015
url http://irep.iium.edu.my/45523/8/Poster_ICBHSR_15_Hanani_4.pdf
http://irep.iium.edu.my/45523/10/Dr_Hanani_2015.pdf
http://irep.iium.edu.my/45523/
https://www.facebook.com/ICBHSR/info?tab=page_info
_version_ 1643612801235681280
score 13.211869

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