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Preparation and characterization of resiquimod-loaded microsponges

INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. M...

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Main Authors: Ab. Hadi, Hazrina, Awadh, Ammar Ihsan JawaD
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/45044/1/45044.pdf
http://irep.iium.edu.my/45044/
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spelling my.iium.irep.450442018-01-03T02:25:01Z http://irep.iium.edu.my/45044/ Preparation and characterization of resiquimod-loaded microsponges Ab. Hadi, Hazrina Awadh, Ammar Ihsan JawaD RS Pharmacy and materia medica INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. MATERIALS AND METHODS 100mg of ethylcellulose and 1mg of resiquimod was dissolved in dichlromethane (DCM) or ethylacetate (EA) and was poured into 1% of polyvinyl alcohol (PVA). The solvent was evaporated in a fume hood. Particle size of microsponges were determined by BT-9300H Laser Particle Size Analyzer. The microspheres were coated with gold palladium alloy and observed with a field emission scanning electron microscope FESEM at an intensity of 10kV. RESULTS AND DISCUSSION With 10 mL DCM with 20 mL PVA 1%, the entrapment efficiency (EE) of resiquimod is 31.32 ± 2.22. The amount of DCM used in the microsponge preparation decreased to 5 mL with 20 mL PVA 1% in which the %EE was found to be high (32.58 ± 1.76%). The preparation with 5 mL EA and 5 mL DCM with 20 mL PVA 1% resulted in lower % EE (23.75 ± 0.30). This might be due to the hardening time of microspheres is faster with EA compared to DCM. The microsponges prepared have homogeneous particle size ranging from 3.82 to 4.21 µm. The surface of microspheres prepared by EA are mainly appeared to be wrinkle, meanwhile microspheres prepared by DCM appeared to have smooth surfaces which indicates rapid polymer precipitation. FigFig. 1. FESEM results of microsponges prepared using DCM in dispersion phase as A) 1 mL, B) 2.5 mL C) 5 mL D) 10 mL. CONCLUSIONS The type and amount of organic solvents play a vital role in the resulted microspheres. The highest % EE and optimum particle size was obtained with low amount of organic solvents. 2015 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/45044/1/45044.pdf Ab. Hadi, Hazrina and Awadh, Ammar Ihsan JawaD (2015) Preparation and characterization of resiquimod-loaded microsponges. In: The 6th APS International PharmSci 2015, 7th-9th Sept. 2015, East Midlands Conference Centre, Nottingham. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Ab. Hadi, Hazrina
Awadh, Ammar Ihsan JawaD
Preparation and characterization of resiquimod-loaded microsponges
description INTRODUCTION Microsponges are porous microspheres, which can be used as a polymeric delivery system to deliver active pharmaceutical ingredient such as resiquimod in a controlled release manner. The objectives of this study were to encapsulate and characterize resiquimod in the microsponges. MATERIALS AND METHODS 100mg of ethylcellulose and 1mg of resiquimod was dissolved in dichlromethane (DCM) or ethylacetate (EA) and was poured into 1% of polyvinyl alcohol (PVA). The solvent was evaporated in a fume hood. Particle size of microsponges were determined by BT-9300H Laser Particle Size Analyzer. The microspheres were coated with gold palladium alloy and observed with a field emission scanning electron microscope FESEM at an intensity of 10kV. RESULTS AND DISCUSSION With 10 mL DCM with 20 mL PVA 1%, the entrapment efficiency (EE) of resiquimod is 31.32 ± 2.22. The amount of DCM used in the microsponge preparation decreased to 5 mL with 20 mL PVA 1% in which the %EE was found to be high (32.58 ± 1.76%). The preparation with 5 mL EA and 5 mL DCM with 20 mL PVA 1% resulted in lower % EE (23.75 ± 0.30). This might be due to the hardening time of microspheres is faster with EA compared to DCM. The microsponges prepared have homogeneous particle size ranging from 3.82 to 4.21 µm. The surface of microspheres prepared by EA are mainly appeared to be wrinkle, meanwhile microspheres prepared by DCM appeared to have smooth surfaces which indicates rapid polymer precipitation. FigFig. 1. FESEM results of microsponges prepared using DCM in dispersion phase as A) 1 mL, B) 2.5 mL C) 5 mL D) 10 mL. CONCLUSIONS The type and amount of organic solvents play a vital role in the resulted microspheres. The highest % EE and optimum particle size was obtained with low amount of organic solvents.
format Conference or Workshop Item
author Ab. Hadi, Hazrina
Awadh, Ammar Ihsan JawaD
author_facet Ab. Hadi, Hazrina
Awadh, Ammar Ihsan JawaD
author_sort Ab. Hadi, Hazrina
title Preparation and characterization of resiquimod-loaded microsponges
title_short Preparation and characterization of resiquimod-loaded microsponges
title_full Preparation and characterization of resiquimod-loaded microsponges
title_fullStr Preparation and characterization of resiquimod-loaded microsponges
title_full_unstemmed Preparation and characterization of resiquimod-loaded microsponges
title_sort preparation and characterization of resiquimod-loaded microsponges
publishDate 2015
url http://irep.iium.edu.my/45044/1/45044.pdf
http://irep.iium.edu.my/45044/
_version_ 1643612700881715200
score 13.211869

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