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Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver

Abstract Oxidative stress plays an important role in cardiovascular diseases. The study investigated the effects of dietary palm tocotrienol-rich fraction on homocysteine metabolism in rats fed a high-methionine diet. Forty-two male Wistar rats were randomly assigned to six groups. Five groups...

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Main Authors: Kamisah, Yusof, Ku Zaifah, Norsidah, Ayob, Azizi, Ahmad Yusof, Asmadi, Othman, Faizah, Nonan, Mohd Rizal, Asmadi, Ahmad Yusof
Format: Article
Language:English
Published: Springer Netherlands 2015
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/44959/1/paper_CBS_2015.pdf
http://irep.iium.edu.my/44959/
http://link.springer.com/article/10.1007%2Fs13105-015-0431-y
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spelling my.iium.irep.449592016-10-20T02:23:50Z http://irep.iium.edu.my/44959/ Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver Kamisah, Yusof Ku Zaifah, Norsidah Ayob, Azizi Ahmad Yusof, Asmadi Othman, Faizah Nonan, Mohd Rizal Asmadi, Ahmad Yusof Q Science (General) Abstract Oxidative stress plays an important role in cardiovascular diseases. The study investigated the effects of dietary palm tocotrienol-rich fraction on homocysteine metabolism in rats fed a high-methionine diet. Forty-two male Wistar rats were randomly assigned to six groups. Five groups were fed with high-methionine diet (1 %) for 10 weeks. Groups 2 to 5 were also given dietary folate (8 mg/kg) and three doses of palm tocotrienol-rich fraction (30, 60 and 150 mg/kg) from week 6 to week 10. The last group was only given basal rat chow. High-methionine diet increased plasma homocysteine after 10 weeks, which was prevented by the supplementations of folate and high-dose palm tocotrienol-rich fraction. Hepatic S-adenosyl methionine (SAM) content was unaffected in all groups but S-adenosyl homocysteine (SAH) content was reduced in the folate group. Folate supplementation increased the SAM/SAH ratio, while in the palm tocotrienol-rich fraction groups, the ratio was lower compared with the folate. Augmented activity of hepatic cystathionine β-synthase and lipid peroxidation content by highmethionine diet was inhibited by palm tocotrienolrich fraction supplementations (moderate and high doses), but not by folate. The supplemented groups had lower hepatic lipid peroxidation than the highmethionine diet. In conclusion, palm tocotrienolrich fraction reduced high-methionine-induced hyperhomocysteinaemia possibly by reducing hepatic oxidative stress in high-methionine-fed rats. It may also exert a direct inhibitory effect on hepatic cystathionine β-synthase. Keywords Tocotrienol . Methionine . Homocysteine . S-Adenosyl methionine . S-Adenosyl homocysteine . Cystathionine β-synthase Springer Netherlands 2015-09-25 Article REM application/pdf en http://irep.iium.edu.my/44959/1/paper_CBS_2015.pdf Kamisah, Yusof and Ku Zaifah, Norsidah and Ayob, Azizi and Ahmad Yusof, Asmadi and Othman, Faizah and Nonan, Mohd Rizal and Asmadi, Ahmad Yusof (2015) Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver. Journal of Physiology and Biochemistry. pp. 1-9. ISSN 1138-7548 http://link.springer.com/article/10.1007%2Fs13105-015-0431-y
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Kamisah, Yusof
Ku Zaifah, Norsidah
Ayob, Azizi
Ahmad Yusof, Asmadi
Othman, Faizah
Nonan, Mohd Rizal
Asmadi, Ahmad Yusof
Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
description Abstract Oxidative stress plays an important role in cardiovascular diseases. The study investigated the effects of dietary palm tocotrienol-rich fraction on homocysteine metabolism in rats fed a high-methionine diet. Forty-two male Wistar rats were randomly assigned to six groups. Five groups were fed with high-methionine diet (1 %) for 10 weeks. Groups 2 to 5 were also given dietary folate (8 mg/kg) and three doses of palm tocotrienol-rich fraction (30, 60 and 150 mg/kg) from week 6 to week 10. The last group was only given basal rat chow. High-methionine diet increased plasma homocysteine after 10 weeks, which was prevented by the supplementations of folate and high-dose palm tocotrienol-rich fraction. Hepatic S-adenosyl methionine (SAM) content was unaffected in all groups but S-adenosyl homocysteine (SAH) content was reduced in the folate group. Folate supplementation increased the SAM/SAH ratio, while in the palm tocotrienol-rich fraction groups, the ratio was lower compared with the folate. Augmented activity of hepatic cystathionine β-synthase and lipid peroxidation content by highmethionine diet was inhibited by palm tocotrienolrich fraction supplementations (moderate and high doses), but not by folate. The supplemented groups had lower hepatic lipid peroxidation than the highmethionine diet. In conclusion, palm tocotrienolrich fraction reduced high-methionine-induced hyperhomocysteinaemia possibly by reducing hepatic oxidative stress in high-methionine-fed rats. It may also exert a direct inhibitory effect on hepatic cystathionine β-synthase. Keywords Tocotrienol . Methionine . Homocysteine . S-Adenosyl methionine . S-Adenosyl homocysteine . Cystathionine β-synthase
format Article
author Kamisah, Yusof
Ku Zaifah, Norsidah
Ayob, Azizi
Ahmad Yusof, Asmadi
Othman, Faizah
Nonan, Mohd Rizal
Asmadi, Ahmad Yusof
author_facet Kamisah, Yusof
Ku Zaifah, Norsidah
Ayob, Azizi
Ahmad Yusof, Asmadi
Othman, Faizah
Nonan, Mohd Rizal
Asmadi, Ahmad Yusof
author_sort Kamisah, Yusof
title Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
title_short Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
title_full Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
title_fullStr Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
title_full_unstemmed Palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
title_sort palm tocotrienol-rich fraction inhibits methionine-induced cystathionine β-synthase in rat liver
publisher Springer Netherlands
publishDate 2015
url http://irep.iium.edu.my/44959/1/paper_CBS_2015.pdf
http://irep.iium.edu.my/44959/
http://link.springer.com/article/10.1007%2Fs13105-015-0431-y
_version_ 1643612683113594880
score 13.211869

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