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In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method

The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis pa...

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Main Authors: Sule, Abubakar, Abd. Samah, Othman, Omar, Muhammad Nor, Ahmed, Qamar Uddin
Format: Conference or Workshop Item
Language:English
Published: 2009
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/4479/1/3.pdf
http://irep.iium.edu.my/4479/
http://pkukmweb.ukm.my/~mspp2009.
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spelling my.iium.irep.44792013-07-17T07:10:42Z http://irep.iium.edu.my/4479/ In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor Ahmed, Qamar Uddin QD Chemistry The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis paniculata (Hempedu Bumi) is used in traditional medicinal practice for the treatment of various chronic and infectious diseases some of bacterial origin2.. This study was carried out to evaluate the antibacterial activity of organic extracts of A. paniculata leaves and stems against 8 pathogenic bacterial strains: Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthrax Micrococcus luteus, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeroginosa and Proteus mirabilis using disc diffusion method. Dried ground powdered leaves and stems were extracted with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and finally with methanol using the soxhlet extraction. The result obtained vividly revealed that the different extracts differed significantly in their antibacterial properties with Dichloromethane extract being the most effective followed by acetone, ethyl acetate, methanol, petroleum ether and lastly ethanol extract. In general, the leaves exhibited strong antibacterial activity than the stems extracts; but all the extracts exerted very good broad spectrum activity against all tested microorganisms. The results explicitly suggest that there might be polar compounds present in the leaves and stems of A. paniculata as the main active ingredients. This finding forms a strong basis for further studies on screening of A. paniculata extracts for the exploration of potent antibacterial agents. 2009-05-12 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/4479/1/3.pdf Sule, Abubakar and Abd. Samah, Othman and Omar, Muhammad Nor and Ahmed, Qamar Uddin (2009) In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method. In: 23rd Malaysian Society of Pharmacology and Physiology Scientific (MSPP) Meeting (Theme: New Technologies for improving quality of life), 12th-13th May 2009, Grand Seasons Hotel, Kuala Lumpur, Malaysia. . http://pkukmweb.ukm.my/~mspp2009.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
Ahmed, Qamar Uddin
In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
description The increasing prevalence in multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies1. The plant Andrographis paniculata (Hempedu Bumi) is used in traditional medicinal practice for the treatment of various chronic and infectious diseases some of bacterial origin2.. This study was carried out to evaluate the antibacterial activity of organic extracts of A. paniculata leaves and stems against 8 pathogenic bacterial strains: Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthrax Micrococcus luteus, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeroginosa and Proteus mirabilis using disc diffusion method. Dried ground powdered leaves and stems were extracted with petroleum ether, dichloromethane, ethyl acetate, acetone, ethanol and finally with methanol using the soxhlet extraction. The result obtained vividly revealed that the different extracts differed significantly in their antibacterial properties with Dichloromethane extract being the most effective followed by acetone, ethyl acetate, methanol, petroleum ether and lastly ethanol extract. In general, the leaves exhibited strong antibacterial activity than the stems extracts; but all the extracts exerted very good broad spectrum activity against all tested microorganisms. The results explicitly suggest that there might be polar compounds present in the leaves and stems of A. paniculata as the main active ingredients. This finding forms a strong basis for further studies on screening of A. paniculata extracts for the exploration of potent antibacterial agents.
format Conference or Workshop Item
author Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
Ahmed, Qamar Uddin
author_facet Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
Ahmed, Qamar Uddin
author_sort Sule, Abubakar
title In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_short In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_full In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_fullStr In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_full_unstemmed In vitro screening of Andrographis paniculata (Hempedu bumi) for antibacterial activity using disc diffusion method
title_sort in vitro screening of andrographis paniculata (hempedu bumi) for antibacterial activity using disc diffusion method
publishDate 2009
url http://irep.iium.edu.my/4479/1/3.pdf
http://irep.iium.edu.my/4479/
http://pkukmweb.ukm.my/~mspp2009.
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score 13.211869

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