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Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans

Enzymatic devulcanisation is one of the methods of recycling waste rubber tire. Among all devulcanised methods that are introduced, biological method is a safer choice because it does not include any harmful or toxic chemicals, and is normally not energy intensive. The process deems more efficien...

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Main Authors: Othman, Emeliana, Yusof, Faridah, Azmi, Azlin Suhaida
Format: Article
Language:English
English
Published: Taylor's University 2015
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/44467/1/P_67.pdf
http://irep.iium.edu.my/44467/4/44467_Application%20of%20Plackett-Burman%20design%20for%20screening%20of%20parameters_SCOPUS.pdf
http://irep.iium.edu.my/44467/
http://jestec.taylors.edu.my/Special%20Issue%203_SOMCHE_2014/SOMCHE%202014_3_2015_012_021.pdf
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spelling my.iium.irep.444672017-11-21T06:37:30Z http://irep.iium.edu.my/44467/ Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans Othman, Emeliana Yusof, Faridah Azmi, Azlin Suhaida TP248.13 Biotechnology Enzymatic devulcanisation is one of the methods of recycling waste rubber tire. Among all devulcanised methods that are introduced, biological method is a safer choice because it does not include any harmful or toxic chemicals, and is normally not energy intensive. The process deems more efficient and less expensive compared to various other devulcanisation methods. This method was done by exposing a surface of a vulcanised rubber piece to at least one enzyme, secreted from chemolithoautotrophic iron- and sulphur-oxidising bacterium to break the sulphur bonds in the vulcanised rubber structure, rendering it more acceptable to be recycled. By applying the enzymatic devulcanisation, it is hoped that the amount of demand of raw rubber can be reduced and at the same time helps to reduce the solid waste disposal problem. In this study, the process parameters that affect the tetrathionate hydrolase activity secreted from Thiobacillus ferrooxidans was screened using PlackettBurman design (PBD). Among the various variables, results showed initial pH, agitation rate, temperature of incubation, inoculum size and calcium chloride concentration were determined as the most influential variables on tetrathionate hydrolase production. Taylor's University 2015-01 Article REM application/pdf en http://irep.iium.edu.my/44467/1/P_67.pdf application/pdf en http://irep.iium.edu.my/44467/4/44467_Application%20of%20Plackett-Burman%20design%20for%20screening%20of%20parameters_SCOPUS.pdf Othman, Emeliana and Yusof, Faridah and Azmi, Azlin Suhaida (2015) Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans. Journal of Engineering Science and Technology, Spec.3. pp. 12-21. ISSN 1823-4690 http://jestec.taylors.edu.my/Special%20Issue%203_SOMCHE_2014/SOMCHE%202014_3_2015_012_021.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Othman, Emeliana
Yusof, Faridah
Azmi, Azlin Suhaida
Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
description Enzymatic devulcanisation is one of the methods of recycling waste rubber tire. Among all devulcanised methods that are introduced, biological method is a safer choice because it does not include any harmful or toxic chemicals, and is normally not energy intensive. The process deems more efficient and less expensive compared to various other devulcanisation methods. This method was done by exposing a surface of a vulcanised rubber piece to at least one enzyme, secreted from chemolithoautotrophic iron- and sulphur-oxidising bacterium to break the sulphur bonds in the vulcanised rubber structure, rendering it more acceptable to be recycled. By applying the enzymatic devulcanisation, it is hoped that the amount of demand of raw rubber can be reduced and at the same time helps to reduce the solid waste disposal problem. In this study, the process parameters that affect the tetrathionate hydrolase activity secreted from Thiobacillus ferrooxidans was screened using PlackettBurman design (PBD). Among the various variables, results showed initial pH, agitation rate, temperature of incubation, inoculum size and calcium chloride concentration were determined as the most influential variables on tetrathionate hydrolase production.
format Article
author Othman, Emeliana
Yusof, Faridah
Azmi, Azlin Suhaida
author_facet Othman, Emeliana
Yusof, Faridah
Azmi, Azlin Suhaida
author_sort Othman, Emeliana
title Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
title_short Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
title_full Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
title_fullStr Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
title_full_unstemmed Application of Plackett-Burman design for screening of parameters for the production of tetrathionate hydrolase by Thiobacillus ferrooxidans
title_sort application of plackett-burman design for screening of parameters for the production of tetrathionate hydrolase by thiobacillus ferrooxidans
publisher Taylor's University
publishDate 2015
url http://irep.iium.edu.my/44467/1/P_67.pdf
http://irep.iium.edu.my/44467/4/44467_Application%20of%20Plackett-Burman%20design%20for%20screening%20of%20parameters_SCOPUS.pdf
http://irep.iium.edu.my/44467/
http://jestec.taylors.edu.my/Special%20Issue%203_SOMCHE_2014/SOMCHE%202014_3_2015_012_021.pdf
_version_ 1643612580168597504
score 13.211869

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