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Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice

The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healt...

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Main Authors: Abdul Ghani, Radiah, Md Yusof, Najwa Farhah, Nik Abdul Malek, Nik Nurasyikin, A.Talib, Norlelawati
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/44455/1/44455.pdf
http://irep.iium.edu.my/44455/
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spelling my.iium.irep.444552018-01-03T01:26:52Z http://irep.iium.edu.my/44455/ Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati RB Pathology The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism. 2015 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/44455/1/44455.pdf Abdul Ghani, Radiah and Md Yusof, Najwa Farhah and Nik Abdul Malek, Nik Nurasyikin and A.Talib, Norlelawati (2015) Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10-12 August 2015, Impiana Resort Cherating, Pahang, Malaysia. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RB Pathology
spellingShingle RB Pathology
Abdul Ghani, Radiah
Md Yusof, Najwa Farhah
Nik Abdul Malek, Nik Nurasyikin
A.Talib, Norlelawati
Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
description The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.
format Conference or Workshop Item
author Abdul Ghani, Radiah
Md Yusof, Najwa Farhah
Nik Abdul Malek, Nik Nurasyikin
A.Talib, Norlelawati
author_facet Abdul Ghani, Radiah
Md Yusof, Najwa Farhah
Nik Abdul Malek, Nik Nurasyikin
A.Talib, Norlelawati
author_sort Abdul Ghani, Radiah
title Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_short Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_full Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_fullStr Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_full_unstemmed Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
title_sort ornithine decarboxylase gene expression in human lung carcinoma cells, a549 treated with pomegranate juice
publishDate 2015
url http://irep.iium.edu.my/44455/1/44455.pdf
http://irep.iium.edu.my/44455/
_version_ 1643612577222098944
score 13.211869

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