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Antioxidant and antibacterial activities of Ruta angustifolia extract

Objectives: To investigate antioxidant properties of R. angustifolia aqueous extract, to evaluate the correlation between polyphenolic contents of the R. angustifolia aqueous extract with the antioxidant activity and to determine the antibacterial activity of R. angustifolia methanolic extract again...

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Main Authors: Muhammad Shuib, Nur Azfa, Mohamed Iqbal, Mohammad Anwar, Sulaiman, Fatimatul Akmal, Razak, Izzatie, Darnis, Deny Susanti
Format: Conference or Workshop Item
Language:English
English
Published: 2015
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/44396/1/44396.pdf
http://irep.iium.edu.my/44396/2/44396a.pdf
http://irep.iium.edu.my/44396/
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spelling my.iium.irep.443962018-12-19T06:36:50Z http://irep.iium.edu.my/44396/ Antioxidant and antibacterial activities of Ruta angustifolia extract Muhammad Shuib, Nur Azfa Mohamed Iqbal, Mohammad Anwar Sulaiman, Fatimatul Akmal Razak, Izzatie Darnis, Deny Susanti QD Chemistry Objectives: To investigate antioxidant properties of R. angustifolia aqueous extract, to evaluate the correlation between polyphenolic contents of the R. angustifolia aqueous extract with the antioxidant activity and to determine the antibacterial activity of R. angustifolia methanolic extract against two Gram positive (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778), two Gram negative (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739). Methods: Aqueous maceration extraction was used for antioxidant activity and methanolic maceration extraction was used for antibacterial activity. The antioxidant properties and activity were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and Beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. Results: Results showed that the phenolic content of R. angustifolia extract was 18.89 g GAE/100g extract while the flavonoid content was 14.170 g QE/100g extract. R. angustifolia exhibited 2.04 mg/mL IC50 for DPPH free radical scavenging activity and 26.38% for average percentage inhibition for beta-carotene bleaching activity. While, the result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Conclusion: Based on the result, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not shown any antibacterial activity. 2015 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/44396/1/44396.pdf application/pdf en http://irep.iium.edu.my/44396/2/44396a.pdf Muhammad Shuib, Nur Azfa and Mohamed Iqbal, Mohammad Anwar and Sulaiman, Fatimatul Akmal and Razak, Izzatie and Darnis, Deny Susanti (2015) Antioxidant and antibacterial activities of Ruta angustifolia extract. In: 5th International Conference on Advancement in Science and Technology (iCAST 2015), 10th-12th August 2015, Impiana Resort Cherating, Kuantan, Pahang. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Muhammad Shuib, Nur Azfa
Mohamed Iqbal, Mohammad Anwar
Sulaiman, Fatimatul Akmal
Razak, Izzatie
Darnis, Deny Susanti
Antioxidant and antibacterial activities of Ruta angustifolia extract
description Objectives: To investigate antioxidant properties of R. angustifolia aqueous extract, to evaluate the correlation between polyphenolic contents of the R. angustifolia aqueous extract with the antioxidant activity and to determine the antibacterial activity of R. angustifolia methanolic extract against two Gram positive (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778), two Gram negative (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739). Methods: Aqueous maceration extraction was used for antioxidant activity and methanolic maceration extraction was used for antibacterial activity. The antioxidant properties and activity were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and Beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. Results: Results showed that the phenolic content of R. angustifolia extract was 18.89 g GAE/100g extract while the flavonoid content was 14.170 g QE/100g extract. R. angustifolia exhibited 2.04 mg/mL IC50 for DPPH free radical scavenging activity and 26.38% for average percentage inhibition for beta-carotene bleaching activity. While, the result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Conclusion: Based on the result, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not shown any antibacterial activity.
format Conference or Workshop Item
author Muhammad Shuib, Nur Azfa
Mohamed Iqbal, Mohammad Anwar
Sulaiman, Fatimatul Akmal
Razak, Izzatie
Darnis, Deny Susanti
author_facet Muhammad Shuib, Nur Azfa
Mohamed Iqbal, Mohammad Anwar
Sulaiman, Fatimatul Akmal
Razak, Izzatie
Darnis, Deny Susanti
author_sort Muhammad Shuib, Nur Azfa
title Antioxidant and antibacterial activities of Ruta angustifolia extract
title_short Antioxidant and antibacterial activities of Ruta angustifolia extract
title_full Antioxidant and antibacterial activities of Ruta angustifolia extract
title_fullStr Antioxidant and antibacterial activities of Ruta angustifolia extract
title_full_unstemmed Antioxidant and antibacterial activities of Ruta angustifolia extract
title_sort antioxidant and antibacterial activities of ruta angustifolia extract
publishDate 2015
url http://irep.iium.edu.my/44396/1/44396.pdf
http://irep.iium.edu.my/44396/2/44396a.pdf
http://irep.iium.edu.my/44396/
_version_ 1643618394102038528
score 13.211869

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