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Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production

The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h...

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Main Authors: Jimat, Dzun Noraini, Azmi, Azlin Suhaida, Ahmad Raus, Raha, Talha, Nur Syakirah
Format: Article
Language:English
Published: Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK) 2015
Subjects:
TP500 Fermentation industries. Beverages. Alcohol
Online Access:http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf
http://irep.iium.edu.my/44096/
http://www.jtrss.org/JTRSS/volume3/UN-44/
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spelling my.iium.irep.440962017-11-23T08:41:16Z http://irep.iium.edu.my/44096/ Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production Jimat, Dzun Noraini Azmi, Azlin Suhaida Ahmad Raus, Raha Talha, Nur Syakirah TP500 Fermentation industries. Beverages. Alcohol The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h followed by destaining with 1 M NaCl solution for 15-20 minutes to observe its cellulolytic activity. One isolates which exposed the largest cellulolytic zone on CMC agar plate was selected for further study. In this study, culture conditions with respect to pH, incubation time, amount of substrate (CPH) and temperature were screened using Design expert version 8.0 by employing two-level factorial design. The selected fungus isolate was cultured in shake flask at 37°C with agitation of 200 rpm for 5 days in incubator shaker. During fermentation period, samples were collected every day for fungal-cellulases activity of filter paper activity (FPase) and carboxymethyl cellulase (CMCase) activity. Analysis of variance (ANOVA) of this study showed that the most significant parameters that affects the production of cellulases from the selected fungi isolates were the amount of substrate (CPH) used followed by the interaction of amount of substrate with pH (p< 0.05). It showed that the cellulases activity was high when the pH 9 with more amount of substrate used. However, it was observed that less significant changes of celllulases activity when different amount of substrate was used at same pH of 3. Based on the microscopic observation of isolate, its morphology was closed to Rhizopus sp.. Further study is essential to optimize the selected culture parameters obtained in this study in order to maximize the activity of cellulases from the selected isolates. Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK) 2015 Article REM application/pdf en http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf Jimat, Dzun Noraini and Azmi, Azlin Suhaida and Ahmad Raus, Raha and Talha, Nur Syakirah (2015) Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production. Journal of Tropical Resources and Sustainable Science (JTRSS), 3. pp. 185-190. ISSN 2289-3946 http://www.jtrss.org/JTRSS/volume3/UN-44/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP500 Fermentation industries. Beverages. Alcohol
spellingShingle TP500 Fermentation industries. Beverages. Alcohol
Jimat, Dzun Noraini
Azmi, Azlin Suhaida
Ahmad Raus, Raha
Talha, Nur Syakirah
Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
description The aim of this study was to screen few fungal isolates from local cocoa pod husks (CPH) which able to secrete cellulases. The isolates were plated on carboxymethyl cellulose (CMC) agar plates which then incubated for two days at 28ºC. Then, these plates were stained with congo red dye for 0.5-1 h followed by destaining with 1 M NaCl solution for 15-20 minutes to observe its cellulolytic activity. One isolates which exposed the largest cellulolytic zone on CMC agar plate was selected for further study. In this study, culture conditions with respect to pH, incubation time, amount of substrate (CPH) and temperature were screened using Design expert version 8.0 by employing two-level factorial design. The selected fungus isolate was cultured in shake flask at 37°C with agitation of 200 rpm for 5 days in incubator shaker. During fermentation period, samples were collected every day for fungal-cellulases activity of filter paper activity (FPase) and carboxymethyl cellulase (CMCase) activity. Analysis of variance (ANOVA) of this study showed that the most significant parameters that affects the production of cellulases from the selected fungi isolates were the amount of substrate (CPH) used followed by the interaction of amount of substrate with pH (p< 0.05). It showed that the cellulases activity was high when the pH 9 with more amount of substrate used. However, it was observed that less significant changes of celllulases activity when different amount of substrate was used at same pH of 3. Based on the microscopic observation of isolate, its morphology was closed to Rhizopus sp.. Further study is essential to optimize the selected culture parameters obtained in this study in order to maximize the activity of cellulases from the selected isolates.
format Article
author Jimat, Dzun Noraini
Azmi, Azlin Suhaida
Ahmad Raus, Raha
Talha, Nur Syakirah
author_facet Jimat, Dzun Noraini
Azmi, Azlin Suhaida
Ahmad Raus, Raha
Talha, Nur Syakirah
author_sort Jimat, Dzun Noraini
title Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_short Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_full Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_fullStr Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_full_unstemmed Screening cellulolytic fungi isolated from Malaysia cocoa pod husk and its culture conditions for cellulases production
title_sort screening cellulolytic fungi isolated from malaysia cocoa pod husk and its culture conditions for cellulases production
publisher Faculty of Agro based Industry (FIAT), Universiti Malaysia Kelantan (UMK)
publishDate 2015
url http://irep.iium.edu.my/44096/1/3-1-185-190_ctress_2014.pdf
http://irep.iium.edu.my/44096/
http://www.jtrss.org/JTRSS/volume3/UN-44/
_version_ 1643612510602919936
score 13.211869

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