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The use of sonication treatment to completely decellularize aorta tissue

We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concen...

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Main Authors: Noor Azmi, Azran Azhim, Yamagami, K., Muramatsu, K., Morimoto, Y., Furukawa, K. S., Tanaka, M., Fukui, Y., Ushida, T.
Format: Article
Language:English
Published: Springer 2013
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf
http://irep.iium.edu.my/44071/
http://dx.doi.org/10.1007/978-3-642-29305-4_522
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spelling my.iium.irep.440712015-08-04T01:14:06Z http://irep.iium.edu.my/44071/ The use of sonication treatment to completely decellularize aorta tissue Noor Azmi, Azran Azhim Yamagami, K. Muramatsu, K. Morimoto, Y. Furukawa, K. S. Tanaka, M. Fukui, Y. Ushida, T. TP248.13 Biotechnology We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, washing treatment after decellularization, SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) and diamidino-2-phenylindol (DAPI) stains. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. There were less than 2.0% of DNA remains after sonication decellularization treatment. Decellularization efficiency increased significantly which treated by sonication in SDS without saline as its DO value smaller compared with treatment in SDS with that. In conclusion, we conclude that sonication treatment is capable to use for preparation of the complete decellularized scaffolds in short treatment time Springer 2013 Article REM application/pdf en http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf Noor Azmi, Azran Azhim and Yamagami, K. and Muramatsu, K. and Morimoto, Y. and Furukawa, K. S. and Tanaka, M. and Fukui, Y. and Ushida, T. (2013) The use of sonication treatment to completely decellularize aorta tissue. World Congress on Medical Physics and Biomedical Engineering, 39. pp. 1987-1990. ISSN 1680-0737 http://dx.doi.org/10.1007/978-3-642-29305-4_522 doi:10.1007/978-3-642-29305-4_522
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Noor Azmi, Azran Azhim
Yamagami, K.
Muramatsu, K.
Morimoto, Y.
Furukawa, K. S.
Tanaka, M.
Fukui, Y.
Ushida, T.
The use of sonication treatment to completely decellularize aorta tissue
description We have developed a novel sonication decellularization system to prepare completely decellularized bioscaffolds in a short treatment time. The aim of the study is to investigate the sonication decellularization efficiency and its relation with ultrasonic power output and dissolved oxygen (DO) concentration in different detergent solution. In the study, we used aorta samples to evaluate sonication decellularization efficiency, which assessed treatment duration, washing treatment after decellularization, SDS detergent with/without saline. The treated samples were evaluated histologically by Hematoxylin Eosin (HE) and diamidino-2-phenylindol (DAPI) stains. The concentration of DO was monitored to identify the effect of sonication on cavitation-related DO concentration in the solution. From histological results, the sonication decellularization efficiency was better than the other preparation methods. There were less than 2.0% of DNA remains after sonication decellularization treatment. Decellularization efficiency increased significantly which treated by sonication in SDS without saline as its DO value smaller compared with treatment in SDS with that. In conclusion, we conclude that sonication treatment is capable to use for preparation of the complete decellularized scaffolds in short treatment time
format Article
author Noor Azmi, Azran Azhim
Yamagami, K.
Muramatsu, K.
Morimoto, Y.
Furukawa, K. S.
Tanaka, M.
Fukui, Y.
Ushida, T.
author_facet Noor Azmi, Azran Azhim
Yamagami, K.
Muramatsu, K.
Morimoto, Y.
Furukawa, K. S.
Tanaka, M.
Fukui, Y.
Ushida, T.
author_sort Noor Azmi, Azran Azhim
title The use of sonication treatment to completely decellularize aorta tissue
title_short The use of sonication treatment to completely decellularize aorta tissue
title_full The use of sonication treatment to completely decellularize aorta tissue
title_fullStr The use of sonication treatment to completely decellularize aorta tissue
title_full_unstemmed The use of sonication treatment to completely decellularize aorta tissue
title_sort use of sonication treatment to completely decellularize aorta tissue
publisher Springer
publishDate 2013
url http://irep.iium.edu.my/44071/1/The_Use_of_Sonication_Treatment_to_Completely_Decellularize_Aorta_Tissue.pdf
http://irep.iium.edu.my/44071/
http://dx.doi.org/10.1007/978-3-642-29305-4_522
_version_ 1643612504805343232
score 13.211869

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