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Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats

Background Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological conse...

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Main Authors: Sayeed, Sayyidah, Saxena, Anil Kumar, Azzubaidi, Marwan Saad, A.Talib, Norlelawati
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
QP Physiology
R Medicine (General)
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
Online Access:http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf
http://irep.iium.edu.my/43795/
http://icadd.org.my/index.php
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spelling my.iium.irep.437952016-05-23T05:32:56Z http://irep.iium.edu.my/43795/ Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats Sayeed, Sayyidah Saxena, Anil Kumar Azzubaidi, Marwan Saad A.Talib, Norlelawati QP Physiology R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM Therapeutics. Pharmacology Background Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological consequences of such a reduction in CBF a similar condition was created in experimental animals. Chronic cerebral hypoperfusion induced neurodegeneration was produced by common carotid artery occlusion (2 vessel occlusion, 2VO) in rats. Since oxidative stress, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was designed to assess the neuroprotective role of Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration. Method After acclimatization, thirty Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-E (treated daily with Vitamin E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. Results There was significant difference in neuronal cell death in 2VO group as compared to sham control group. In Vitamin E-treated 2VO (2VO-E) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group. Conclusion Vitamin E is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be effectively utilized in the management of AD. The antioxidant activity of Vitamin E is in progress and will be evaluated by the measurement of Isoprostane F2 through Enzyme-Linked Immunosorbent Assay. 2015-06-03 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf Sayeed, Sayyidah and Saxena, Anil Kumar and Azzubaidi, Marwan Saad and A.Talib, Norlelawati (2015) Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats. In: International Conference of Antioxidants and Degenerative Diseases (ICADD 2015), 3-4th June 2015, Hotel Istana, Kuala Lumpur, Malaysia. http://icadd.org.my/index.php
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QP Physiology
R Medicine (General)
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
spellingShingle QP Physiology
R Medicine (General)
RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM Therapeutics. Pharmacology
Sayeed, Sayyidah
Saxena, Anil Kumar
Azzubaidi, Marwan Saad
A.Talib, Norlelawati
Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
description Background Chronic cerebral hypoperfusion has been linked with cognitive decline in aging and Alzheimer's disease (AD). A reduced cerebral blood flow (CBF) is considered to be a predictive marker for the progression into Alzheimer's disease. In order to tackle the neuropathological consequences of such a reduction in CBF a similar condition was created in experimental animals. Chronic cerebral hypoperfusion induced neurodegeneration was produced by common carotid artery occlusion (2 vessel occlusion, 2VO) in rats. Since oxidative stress, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was designed to assess the neuroprotective role of Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration. Method After acclimatization, thirty Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A – sham control, Group B – 2VO, and Group C – 2VO-E (treated daily with Vitamin E, 100 mg/kg, orally following 2VO). On the 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA-1 region was estimated. Results There was significant difference in neuronal cell death in 2VO group as compared to sham control group. In Vitamin E-treated 2VO (2VO-E) rats, the viable neuronal cell count of the hippocampal CA-1 region was significantly higher as compared to the untreated 2VO group. Conclusion Vitamin E is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats and can be effectively utilized in the management of AD. The antioxidant activity of Vitamin E is in progress and will be evaluated by the measurement of Isoprostane F2 through Enzyme-Linked Immunosorbent Assay.
format Conference or Workshop Item
author Sayeed, Sayyidah
Saxena, Anil Kumar
Azzubaidi, Marwan Saad
A.Talib, Norlelawati
author_facet Sayeed, Sayyidah
Saxena, Anil Kumar
Azzubaidi, Marwan Saad
A.Talib, Norlelawati
author_sort Sayeed, Sayyidah
title Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_short Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_full Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_fullStr Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_full_unstemmed Assessment of ROS status and neuroprotection by Vitamin E in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_sort assessment of ros status and neuroprotection by vitamin e in chronic cerebral hypoperfusion-induced neurodegeneration in rats
publishDate 2015
url http://irep.iium.edu.my/43795/1/CONFERENCE_2015_Sayyidah.pdf
http://irep.iium.edu.my/43795/
http://icadd.org.my/index.php
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score 13.211869

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