Staff View: Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor

Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell num...

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Main Authors:	Arifin, Mohd Azmir, Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Ideris, Aini
Format:	Article
Language:	English
Published:	Hindawi Publishing Corporation 2010
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol
Online Access:	http://irep.iium.edu.my/4244/1/586363-JBB_Azmir_Paper.pdf http://irep.iium.edu.my/4244/
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spelling	my.iium.irep.42442011-09-21T03:18:36Z http://irep.iium.edu.my/4244/ Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor Arifin, Mohd Azmir Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini TP155 Chemical engineering TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco’s Modified Eagle Medium (DMEM) which was 1.93 × 106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about 7.95 × 105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on 3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that themaximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation. Hindawi Publishing Corporation 2010 Article REM application/pdf en http://irep.iium.edu.my/4244/1/586363-JBB_Azmir_Paper.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Ideris, Aini (2010) Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor. Journal of Biomedicine and Biotechnology, 2010 (586363). pp. 1-7. ISSN 1110-7243 doi:10.1155/2010/586363 doi:10.1155/2010/586363
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol Arifin, Mohd Azmir Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
description	The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco’s Modified Eagle Medium (DMEM) which was 1.93 × 106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about 7.95 × 105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on 3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that themaximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.
format	Article
author	Arifin, Mohd Azmir Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini
author_facet	Arifin, Mohd Azmir Mel, Maizirwan Abdul Karim, Mohamed Ismail Ideris, Aini
author_sort	Arifin, Mohd Azmir
title	Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
title_short	Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
title_full	Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
title_fullStr	Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
title_full_unstemmed	Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
title_sort	production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor
publisher	Hindawi Publishing Corporation
publishDate	2010
url	http://irep.iium.edu.my/4244/1/586363-JBB_Azmir_Paper.pdf http://irep.iium.edu.my/4244/
_version_	1643605302718758912
score	13.211869

Production of newcastle disease virus by vero cells grown on cytodex 1 microcarriers in a 2-litre stirred tank bioreactor

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