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Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell

We investigate the influence of buffer electrolyte and pH on the electrochemical performance of glucose oxidase-laccase biofuel cell (BFC). A simplified system design is adopted i.e. we employ freely-suspended glucose oxidase and laccase enzymes. The BFC comprises nickel mesh as the oxidative curren...

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Main Authors: Asrul, Nurrisa, Othman, Raihan, Mohd. Salleh, Hamzah, Ali, Mohammad Nazri
Format: Article
Language:English
Published: American Scientific Publishers 2013
Subjects:
TA164 Bioengineering
TJ807 Renewable energy sources
TP248.13 Biotechnology
TP250 Industrial electrochemistry
Online Access:http://irep.iium.edu.my/42406/1/J_Biobased_Mat.pdf
http://irep.iium.edu.my/42406/
http://www.aspbs.com/jbmbe/contents_jbmbe2013.htm#v7n2
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spelling my.iium.irep.424062021-07-14T09:45:31Z http://irep.iium.edu.my/42406/ Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell Asrul, Nurrisa Othman, Raihan Mohd. Salleh, Hamzah Ali, Mohammad Nazri TA164 Bioengineering TJ807 Renewable energy sources TP248.13 Biotechnology TP250 Industrial electrochemistry We investigate the influence of buffer electrolyte and pH on the electrochemical performance of glucose oxidase-laccase biofuel cell (BFC). A simplified system design is adopted i.e. we employ freely-suspended glucose oxidase and laccase enzymes. The BFC comprises nickel mesh as the oxidative current collector and a carbon-based air electrode as the reductive current collector, enclosed in acrylic casing of 3 ml volumetric capacity. The air electrode also serves as the ambient oxygen diffusion site to continuously feed oxygen into the system. Three types of anolyte/catholyte buffer electrolyte are studied – citrate/citrate, phosphate/phosphate and citrate/phosphate, in the pH range 5 – 6.5. A biocatalytic electrochemical system is highly sensitive. Consequently, any variation in the electrolyte formulation would affect the cell discharge performance. Thus, we utilize the discharge profile capacity of the BFC to elucidate the optimum electrolyte formulation. Though the approach is indirect, the observed changes are obvious, suggesting the method is viable. It is found that the citrate/citrate buffer electrolyte of pH 5 exhibits the highest power output of 1074 mWh or volumetric power density of 1.7 Wh/l. The cell is able to sustain continuous discharge current of 30µA for about 31.75 hours with operating voltage around 1.0 V, in an uncontrolled open environment. Interestingly, the observed discharge performance and power density are comparable to BFC employing immobilized enzymes and mediators, in a controlled environment. American Scientific Publishers 2013-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42406/1/J_Biobased_Mat.pdf Asrul, Nurrisa and Othman, Raihan and Mohd. Salleh, Hamzah and Ali, Mohammad Nazri (2013) Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell. Journal of Biobased Materials and Bioenergy, 7 (2). pp. 194-197. ISSN 1556-6560 http://www.aspbs.com/jbmbe/contents_jbmbe2013.htm#v7n2 10.1166/jbmb.2013.1337
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TA164 Bioengineering
TJ807 Renewable energy sources
TP248.13 Biotechnology
TP250 Industrial electrochemistry
spellingShingle TA164 Bioengineering
TJ807 Renewable energy sources
TP248.13 Biotechnology
TP250 Industrial electrochemistry
Asrul, Nurrisa
Othman, Raihan
Mohd. Salleh, Hamzah
Ali, Mohammad Nazri
Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
description We investigate the influence of buffer electrolyte and pH on the electrochemical performance of glucose oxidase-laccase biofuel cell (BFC). A simplified system design is adopted i.e. we employ freely-suspended glucose oxidase and laccase enzymes. The BFC comprises nickel mesh as the oxidative current collector and a carbon-based air electrode as the reductive current collector, enclosed in acrylic casing of 3 ml volumetric capacity. The air electrode also serves as the ambient oxygen diffusion site to continuously feed oxygen into the system. Three types of anolyte/catholyte buffer electrolyte are studied – citrate/citrate, phosphate/phosphate and citrate/phosphate, in the pH range 5 – 6.5. A biocatalytic electrochemical system is highly sensitive. Consequently, any variation in the electrolyte formulation would affect the cell discharge performance. Thus, we utilize the discharge profile capacity of the BFC to elucidate the optimum electrolyte formulation. Though the approach is indirect, the observed changes are obvious, suggesting the method is viable. It is found that the citrate/citrate buffer electrolyte of pH 5 exhibits the highest power output of 1074 mWh or volumetric power density of 1.7 Wh/l. The cell is able to sustain continuous discharge current of 30µA for about 31.75 hours with operating voltage around 1.0 V, in an uncontrolled open environment. Interestingly, the observed discharge performance and power density are comparable to BFC employing immobilized enzymes and mediators, in a controlled environment.
format Article
author Asrul, Nurrisa
Othman, Raihan
Mohd. Salleh, Hamzah
Ali, Mohammad Nazri
author_facet Asrul, Nurrisa
Othman, Raihan
Mohd. Salleh, Hamzah
Ali, Mohammad Nazri
author_sort Asrul, Nurrisa
title Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
title_short Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
title_full Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
title_fullStr Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
title_full_unstemmed Influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
title_sort influence of buffer electrolyte and ph on the electrochemical performance of glucose oxidase-laccase biofuel cell
publisher American Scientific Publishers
publishDate 2013
url http://irep.iium.edu.my/42406/1/J_Biobased_Mat.pdf
http://irep.iium.edu.my/42406/
http://www.aspbs.com/jbmbe/contents_jbmbe2013.htm#v7n2
_version_ 1706956531843465216
score 13.211869

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