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Optimisation of hydrolysis conditions for ethanol production from sorghum starch

The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of s...

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Main Authors: Nadir, Najiah, Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Mohd Yunus, Rosli
Format: Article
Language:English
Published: The Institution of Engineers 2010
Subjects:
TP155 Chemical engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf
http://irep.iium.edu.my/4240/
http://www.myiem.org.my/content/iem_journal_2010-181.aspx
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spelling my.iium.irep.42402011-09-22T07:32:16Z http://irep.iium.edu.my/4240/ Optimisation of hydrolysis conditions for ethanol production from sorghum starch Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli TP155 Chemical engineering TP248.13 Biotechnology The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. The Institution of Engineers 2010-09 Article REM application/pdf en http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf Nadir, Najiah and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yunus, Rosli (2010) Optimisation of hydrolysis conditions for ethanol production from sorghum starch. Journal - The Institution of Engineers, Malaysia, 71 (3). pp. 26-34. ISSN 0126-513X http://www.myiem.org.my/content/iem_journal_2010-181.aspx
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
Nadir, Najiah
Mel, Maizirwan
Abdul Karim, Mohamed Ismail
Mohd Yunus, Rosli
Optimisation of hydrolysis conditions for ethanol production from sorghum starch
description The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme.
format Article
author Nadir, Najiah
Mel, Maizirwan
Abdul Karim, Mohamed Ismail
Mohd Yunus, Rosli
author_facet Nadir, Najiah
Mel, Maizirwan
Abdul Karim, Mohamed Ismail
Mohd Yunus, Rosli
author_sort Nadir, Najiah
title Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_short Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_fullStr Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_full_unstemmed Optimisation of hydrolysis conditions for ethanol production from sorghum starch
title_sort optimisation of hydrolysis conditions for ethanol production from sorghum starch
publisher The Institution of Engineers
publishDate 2010
url http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf
http://irep.iium.edu.my/4240/
http://www.myiem.org.my/content/iem_journal_2010-181.aspx
_version_ 1643605301258092544
score 13.211869

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