Study of cells attachment using impedance spectroscopy technique
In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwel...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4226/1/ASME2011.pdf http://irep.iium.edu.my/4226/ http://www.asmeconferences.org/congress2011/ |
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| Summary: | In this paper we report on the electrical impedance
spectroscopy characterization of the attachment of two
mammalian cell lines: endothelial cells (RFPEC) and fibroblast
cells (V79). For electrical cell–substrate impedance sensing
(ECIS) of the endothelial cells a commercially available eightwell
cell culture impedance array (ECIS-8W1E) was used. The
impedance measurements were recorded with cell culture
medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL MultiphysicsTM, a commercially available modeling package. |
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