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Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold

Previously, we have proven that fibrin and poly(lactic-co-glycolic acid) (PLGA) scaffolds facilitate cell proliferation, matrix production and early chondrogenesis of rabbit articular chondrocytes in in vitro and in vivo experiments. In this study, we evaluated the potential of fibrin/PLGA scaffold...

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Main Authors: Sha'ban, Munirah, Yoon, Sun Jung, Ko, Youn Kyung, Ha, Hyun Jang, Kim, Soon Hee, So, Jung Won, Idrus, Ruszymah, Khang, Gilson
Format: Article
Language:English
Published: Taylor & Francis Group 2008
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/41858/1/JBSPE_2008_MUNIRAH_ET_AL.pdf
http://irep.iium.edu.my/41858/
http://www.tandfonline.com/toc/tbsp20/current#.VS8XifmSxqV
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spelling my.iium.irep.418582015-04-16T01:48:40Z http://irep.iium.edu.my/41858/ Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold Sha'ban, Munirah Yoon, Sun Jung Ko, Youn Kyung Ha, Hyun Jang Kim, Soon Hee So, Jung Won Idrus, Ruszymah Khang, Gilson R Medicine (General) Previously, we have proven that fibrin and poly(lactic-co-glycolic acid) (PLGA) scaffolds facilitate cell proliferation, matrix production and early chondrogenesis of rabbit articular chondrocytes in in vitro and in vivo experiments. In this study, we evaluated the potential of fibrin/PLGA scaffold for intervertebral disc (IVD) tissue engineering using annulus fibrosus (AF) and nucleus pulposus (NP) cells in relation to potential clinical application. PLGA scaffolds were soaked in cells–fibrin suspension and polymerized by dropping thrombin–sodium chloride (CaCl2) solution. A PLGA–cell complex without fibrin was used as control. Higher cellular proliferation activity was observed in fibrin/PLGA-seeded AF and NP cells at each time point of 3, 7, 14 and 7 days using the MTT assay. After 3 weeks in vitro incubation, fibrin/PLGA exhibited a firmer gross morphology than PLGA groups. A significant cartilaginous tissue formation was observed in fibrin/PLGA, as proven by the development of cells cluster of various sizes and three-dimensional (3D) cartilaginous histoarchitecture and the presence of proteoglycan-rich matrix and glycosaminoglycan (GAG). The sGAG production measured by 1,9-dimethylmethylene blue (DMMB) assay revealed greater sGAG production in fibrin/PLGA than PLGA group. Immunohistochemical analyses showed expressions of collagen type II, aggrecan core protein and collagen type I genes throughout in vitro culture in both fibrin/PLGA and PLGA. In conclusion, fibrin promotes cell proliferation, stable in vitro tissue morphology, superior cartilaginous tissue formation and sGAG production of AF and NP cells cultured in PLGA scaffold. The 3D porous PLGA scaffold–cell complexes using fibrin can provide a vehicle for delivery of cells to regenerate tissue-engineered IVD tissue. Taylor & Francis Group 2008 Article REM application/pdf en http://irep.iium.edu.my/41858/1/JBSPE_2008_MUNIRAH_ET_AL.pdf Sha'ban, Munirah and Yoon, Sun Jung and Ko, Youn Kyung and Ha, Hyun Jang and Kim, Soon Hee and So, Jung Won and Idrus, Ruszymah and Khang, Gilson (2008) Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold. Journal of Biomaterials Science, Polymer Edition, 19 (9). pp. 1219-1237. ISSN 0920-5063 (Print), 1568-5624 (Online) http://www.tandfonline.com/toc/tbsp20/current#.VS8XifmSxqV
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Sha'ban, Munirah
Yoon, Sun Jung
Ko, Youn Kyung
Ha, Hyun Jang
Kim, Soon Hee
So, Jung Won
Idrus, Ruszymah
Khang, Gilson
Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
description Previously, we have proven that fibrin and poly(lactic-co-glycolic acid) (PLGA) scaffolds facilitate cell proliferation, matrix production and early chondrogenesis of rabbit articular chondrocytes in in vitro and in vivo experiments. In this study, we evaluated the potential of fibrin/PLGA scaffold for intervertebral disc (IVD) tissue engineering using annulus fibrosus (AF) and nucleus pulposus (NP) cells in relation to potential clinical application. PLGA scaffolds were soaked in cells–fibrin suspension and polymerized by dropping thrombin–sodium chloride (CaCl2) solution. A PLGA–cell complex without fibrin was used as control. Higher cellular proliferation activity was observed in fibrin/PLGA-seeded AF and NP cells at each time point of 3, 7, 14 and 7 days using the MTT assay. After 3 weeks in vitro incubation, fibrin/PLGA exhibited a firmer gross morphology than PLGA groups. A significant cartilaginous tissue formation was observed in fibrin/PLGA, as proven by the development of cells cluster of various sizes and three-dimensional (3D) cartilaginous histoarchitecture and the presence of proteoglycan-rich matrix and glycosaminoglycan (GAG). The sGAG production measured by 1,9-dimethylmethylene blue (DMMB) assay revealed greater sGAG production in fibrin/PLGA than PLGA group. Immunohistochemical analyses showed expressions of collagen type II, aggrecan core protein and collagen type I genes throughout in vitro culture in both fibrin/PLGA and PLGA. In conclusion, fibrin promotes cell proliferation, stable in vitro tissue morphology, superior cartilaginous tissue formation and sGAG production of AF and NP cells cultured in PLGA scaffold. The 3D porous PLGA scaffold–cell complexes using fibrin can provide a vehicle for delivery of cells to regenerate tissue-engineered IVD tissue.
format Article
author Sha'ban, Munirah
Yoon, Sun Jung
Ko, Youn Kyung
Ha, Hyun Jang
Kim, Soon Hee
So, Jung Won
Idrus, Ruszymah
Khang, Gilson
author_facet Sha'ban, Munirah
Yoon, Sun Jung
Ko, Youn Kyung
Ha, Hyun Jang
Kim, Soon Hee
So, Jung Won
Idrus, Ruszymah
Khang, Gilson
author_sort Sha'ban, Munirah
title Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
title_short Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
title_full Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
title_fullStr Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
title_full_unstemmed Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
title_sort fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold
publisher Taylor & Francis Group
publishDate 2008
url http://irep.iium.edu.my/41858/1/JBSPE_2008_MUNIRAH_ET_AL.pdf
http://irep.iium.edu.my/41858/
http://www.tandfonline.com/toc/tbsp20/current#.VS8XifmSxqV
_version_ 1643612090282278912
score 13.211869

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