صورة الغلاف

Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold

INTRODUCTION: The incorporation of fibrin onto synthetic poly (lactic-co-glycolic acid) or PLGA scaffold allows efficient cell attachment, proliferation, differentiation and migration. The study aimed to compare between direct pipetting and centrifugation techniques for the incorporation of fibrin o...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Mohamad, Mohd Yusof, Md Nazir, Noorhidayah, Abdul Rahman, Rozlin, Mohamad Sukri, Norhamiza, Ahmad Radzi, Muhammad Aa'zamuddin, Sha'ban, Munirah
التنسيق: Conference or Workshop Item
اللغة:English
منشور في: 2014
الموضوعات:
R Medicine (General)
الوصول للمادة أونلاين:http://irep.iium.edu.my/41795/1/KAHS_RW2014_YUSOF.pdf
http://irep.iium.edu.my/41795/
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spelling my.iium.irep.417952015-02-18T04:51:26Z http://irep.iium.edu.my/41795/ Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold Mohamad, Mohd Yusof Md Nazir, Noorhidayah Abdul Rahman, Rozlin Mohamad Sukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Sha'ban, Munirah R Medicine (General) INTRODUCTION: The incorporation of fibrin onto synthetic poly (lactic-co-glycolic acid) or PLGA scaffold allows efficient cell attachment, proliferation, differentiation and migration. The study aimed to compare between direct pipetting and centrifugation techniques for the incorporation of fibrin onto PLGA. MATERIALS AND METHODS: Poly lactic-co-glycolic acid is fabricated via solvent casting, salt leaching technique. Sieved sodium chloride (NaCl) particles (approx.400μm) were dispersed in a polymer/solvent solution (0.2% w/v PLGA/methylene chloride). The mixture was casted into cylindrical disk scaffold using silicone moulds (7 mm in diameter and 3 mm in thickness). The scaffolds were immersed in deionized water for 48 hours to leach out NaCl and then, freeze-dried. Fibrin was derived from the whole blood via centrifugation at 4800 rpm, 5minutes, 4°C. The resulting supernatant was filter-sterilized and stored at -20°C until use. Direct pipetting method involves pipetting approx. 100 µl of fibrin on the surface of PLGA. Centrifugation method involved dipping of PLGA scaffold in 1 ml of fibrin and centrifugation at 5600 rpm for 10 minutes. Both scaffolds for each method were polymerized by dropping calcium chloride solution. The scaffolds were stored at room temperature and analysed using scanning electron microscopy. RESULTS AND DISCUSSION: The results indicated that the centrifugation shows better fibrin incorporation inside the PLGA pores when compared to the direct pipetting method. CONCLUSION: This easy centrifugation procedure may facilitate the establishment of efficient cell delivery system for tissue engineering. 2014 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/41795/1/KAHS_RW2014_YUSOF.pdf Mohamad, Mohd Yusof and Md Nazir, Noorhidayah and Abdul Rahman, Rozlin and Mohamad Sukri, Norhamiza and Ahmad Radzi, Muhammad Aa'zamuddin and Sha'ban, Munirah (2014) Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold. In: K.A.H.S. Research Week 2014, 17th-21st November 2014, Kulliyyah of Allied Health Sciences, IIUM. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mohamad, Mohd Yusof
Md Nazir, Noorhidayah
Abdul Rahman, Rozlin
Mohamad Sukri, Norhamiza
Ahmad Radzi, Muhammad Aa'zamuddin
Sha'ban, Munirah
Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
description INTRODUCTION: The incorporation of fibrin onto synthetic poly (lactic-co-glycolic acid) or PLGA scaffold allows efficient cell attachment, proliferation, differentiation and migration. The study aimed to compare between direct pipetting and centrifugation techniques for the incorporation of fibrin onto PLGA. MATERIALS AND METHODS: Poly lactic-co-glycolic acid is fabricated via solvent casting, salt leaching technique. Sieved sodium chloride (NaCl) particles (approx.400μm) were dispersed in a polymer/solvent solution (0.2% w/v PLGA/methylene chloride). The mixture was casted into cylindrical disk scaffold using silicone moulds (7 mm in diameter and 3 mm in thickness). The scaffolds were immersed in deionized water for 48 hours to leach out NaCl and then, freeze-dried. Fibrin was derived from the whole blood via centrifugation at 4800 rpm, 5minutes, 4°C. The resulting supernatant was filter-sterilized and stored at -20°C until use. Direct pipetting method involves pipetting approx. 100 µl of fibrin on the surface of PLGA. Centrifugation method involved dipping of PLGA scaffold in 1 ml of fibrin and centrifugation at 5600 rpm for 10 minutes. Both scaffolds for each method were polymerized by dropping calcium chloride solution. The scaffolds were stored at room temperature and analysed using scanning electron microscopy. RESULTS AND DISCUSSION: The results indicated that the centrifugation shows better fibrin incorporation inside the PLGA pores when compared to the direct pipetting method. CONCLUSION: This easy centrifugation procedure may facilitate the establishment of efficient cell delivery system for tissue engineering.
format Conference or Workshop Item
author Mohamad, Mohd Yusof
Md Nazir, Noorhidayah
Abdul Rahman, Rozlin
Mohamad Sukri, Norhamiza
Ahmad Radzi, Muhammad Aa'zamuddin
Sha'ban, Munirah
author_facet Mohamad, Mohd Yusof
Md Nazir, Noorhidayah
Abdul Rahman, Rozlin
Mohamad Sukri, Norhamiza
Ahmad Radzi, Muhammad Aa'zamuddin
Sha'ban, Munirah
author_sort Mohamad, Mohd Yusof
title Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
title_short Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
title_full Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
title_fullStr Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
title_full_unstemmed Centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
title_sort centrifugation facilitates incorporation of fibrin in poly(lactic-co-glycolic acid) scaffold
publishDate 2014
url http://irep.iium.edu.my/41795/1/KAHS_RW2014_YUSOF.pdf
http://irep.iium.edu.my/41795/
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score 13.25181

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