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Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering

Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essent...

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Main Authors: Ahmad Radzi, Muhammad Aa'zamuddin, Jamaludin, Fatin Nabila, Abdul Rahman, Rozlin, Md Nazir, Noorhidayah, Mohamad Sukri, Norhamiza, Mohammad, Mohd Yusof, Zulkifly, Ahmad Hafiz, Che Ahmad, Aminudin, Abdul Rahman, Suzanah, Hashi, Abdurezak Abdulahi, Sha'ban, Munirah
Format: Article
Language:English
Published: Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/41425/1/RR-181114-068.pdf
http://irep.iium.edu.my/41425/
http://regres.tesma.org.my/pdf/RR-181114-068.pdf
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spelling my.iium.irep.414252015-02-12T08:09:19Z http://irep.iium.edu.my/41425/ Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering Ahmad Radzi, Muhammad Aa'zamuddin Jamaludin, Fatin Nabila Abdul Rahman, Rozlin Md Nazir, Noorhidayah Mohamad Sukri, Norhamiza Mohammad, Mohd Yusof Zulkifly, Ahmad Hafiz Che Ahmad, Aminudin Abdul Rahman, Suzanah Hashi, Abdurezak Abdulahi Sha'ban, Munirah R Medicine (General) Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essential Medium (α-MEM) and, (2) the mixture of Ham F12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (FD). Growth kinetics assessment used in this study includes cell viability, total cell yield, growth rate, population doubling time and total number of cell doubling. FD was found to be an appropriate culture medium to promote better BMMSC growth. BMMSC from iliac crest region showed better proliferation potential than the femur region. Hence, it can be inferred from this study that BMMSC harvested from the iliac crest site and cultured in FD medium is better than femur samples in terms of growth profile. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article REM application/pdf en http://irep.iium.edu.my/41425/1/RR-181114-068.pdf Ahmad Radzi, Muhammad Aa'zamuddin and Jamaludin, Fatin Nabila and Abdul Rahman, Rozlin and Md Nazir, Noorhidayah and Mohamad Sukri, Norhamiza and Mohammad, Mohd Yusof and Zulkifly, Ahmad Hafiz and Che Ahmad, Aminudin and Abdul Rahman, Suzanah and Hashi, Abdurezak Abdulahi and Sha'ban, Munirah (2014) Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering. Regenerative Research, 3 (2). pp. 118-119. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-068.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Ahmad Radzi, Muhammad Aa'zamuddin
Jamaludin, Fatin Nabila
Abdul Rahman, Rozlin
Md Nazir, Noorhidayah
Mohamad Sukri, Norhamiza
Mohammad, Mohd Yusof
Zulkifly, Ahmad Hafiz
Che Ahmad, Aminudin
Abdul Rahman, Suzanah
Hashi, Abdurezak Abdulahi
Sha'ban, Munirah
Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
description Stem cell has been widely researched during the last decade due to its potential to differentiate into many cell types. This study attempts to compare the growth of bone marrow mesenchymal stem cells (BMMSC) aspirated from rabbits’ iliac crest and femur in two culture media i.e. (1) α-Minimum Essential Medium (α-MEM) and, (2) the mixture of Ham F12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (FD). Growth kinetics assessment used in this study includes cell viability, total cell yield, growth rate, population doubling time and total number of cell doubling. FD was found to be an appropriate culture medium to promote better BMMSC growth. BMMSC from iliac crest region showed better proliferation potential than the femur region. Hence, it can be inferred from this study that BMMSC harvested from the iliac crest site and cultured in FD medium is better than femur samples in terms of growth profile.
format Article
author Ahmad Radzi, Muhammad Aa'zamuddin
Jamaludin, Fatin Nabila
Abdul Rahman, Rozlin
Md Nazir, Noorhidayah
Mohamad Sukri, Norhamiza
Mohammad, Mohd Yusof
Zulkifly, Ahmad Hafiz
Che Ahmad, Aminudin
Abdul Rahman, Suzanah
Hashi, Abdurezak Abdulahi
Sha'ban, Munirah
author_facet Ahmad Radzi, Muhammad Aa'zamuddin
Jamaludin, Fatin Nabila
Abdul Rahman, Rozlin
Md Nazir, Noorhidayah
Mohamad Sukri, Norhamiza
Mohammad, Mohd Yusof
Zulkifly, Ahmad Hafiz
Che Ahmad, Aminudin
Abdul Rahman, Suzanah
Hashi, Abdurezak Abdulahi
Sha'ban, Munirah
author_sort Ahmad Radzi, Muhammad Aa'zamuddin
title Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_short Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_full Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_fullStr Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_full_unstemmed Evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
title_sort evaluation of bone marrow mesenchymal stem cells growth in vitro for cartilage tissue engineering
publisher Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate 2014
url http://irep.iium.edu.my/41425/1/RR-181114-068.pdf
http://irep.iium.edu.my/41425/
http://regres.tesma.org.my/pdf/RR-181114-068.pdf
_version_ 1643612021535539200
score 13.211869

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