Cover Image

Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface

The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The...

Full description

Saved in:
Bibliographic Details
Main Authors: Ismail, Rozila, Tan, A. W., Pingguan-Murphy, Belinda, Sha'ban, Munirah, Chua, Kien Hui
Format: Article
Language:English
Published: Tissue Engineering and Regenerative Medicine Society of Malaysia 2014
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/41424/1/RR-181114-041.pdf
http://irep.iium.edu.my/41424/
http://regres.tesma.org.my/pdf/RR-181114-041.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.41424
record_format dspace
spelling my.iium.irep.414242015-02-12T09:11:39Z http://irep.iium.edu.my/41424/ Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface Ismail, Rozila Tan, A. W. Pingguan-Murphy, Belinda Sha'ban, Munirah Chua, Kien Hui R Medicine (General) The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The non-coated TiO2 was used as control. The effects of TiO2 based scaffolds on cell adhesion were characterized by scanning electron microscopy (SEM). Cell viability, differentiation and mineralization were assessed by Alamar Blue, alkaline phosphatase (ALP) and Alizarin Red assays, respectively. The combination of HADSC/HOB, 2:1 ratio, seeded on nanofibrous-coated TiO2 showed better cell adhesion, viability, differentiation and mineralization than the other groups. This study offers opportunity to assess in vitro cellular development of HADSC through direct cell to cell contact with HOB. This study indicates that the co-cultured HADSC/HOB seeded on TiO2 based scaffolds may serve as a promising approach to facilitate osteogenic differentiation activity. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article REM application/pdf en http://irep.iium.edu.my/41424/1/RR-181114-041.pdf Ismail, Rozila and Tan, A. W. and Pingguan-Murphy, Belinda and Sha'ban, Munirah and Chua, Kien Hui (2014) Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface. Regenerative Research, 3 (2). pp. 65-66. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-041.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Ismail, Rozila
Tan, A. W.
Pingguan-Murphy, Belinda
Sha'ban, Munirah
Chua, Kien Hui
Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
description The study aims to evaluate the osteogenic potential of human adipose derived stem cell (HADSC) co-culture with human osteoblast (HOB) using selected HADSC/HOB ratio of 2:1, 1:1 and 1:2, respectively. The HADSC/HOB was seeded on Titanium dioxide (TiO2) coated with or without nanofibre substrate. The non-coated TiO2 was used as control. The effects of TiO2 based scaffolds on cell adhesion were characterized by scanning electron microscopy (SEM). Cell viability, differentiation and mineralization were assessed by Alamar Blue, alkaline phosphatase (ALP) and Alizarin Red assays, respectively. The combination of HADSC/HOB, 2:1 ratio, seeded on nanofibrous-coated TiO2 showed better cell adhesion, viability, differentiation and mineralization than the other groups. This study offers opportunity to assess in vitro cellular development of HADSC through direct cell to cell contact with HOB. This study indicates that the co-cultured HADSC/HOB seeded on TiO2 based scaffolds may serve as a promising approach to facilitate osteogenic differentiation activity.
format Article
author Ismail, Rozila
Tan, A. W.
Pingguan-Murphy, Belinda
Sha'ban, Munirah
Chua, Kien Hui
author_facet Ismail, Rozila
Tan, A. W.
Pingguan-Murphy, Belinda
Sha'ban, Munirah
Chua, Kien Hui
author_sort Ismail, Rozila
title Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_short Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_full Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_fullStr Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_full_unstemmed Osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
title_sort osteogenic potential of human adipose derived stem cell co-culture with human osteoblast on titanium dioxide nanofibrous surface
publisher Tissue Engineering and Regenerative Medicine Society of Malaysia
publishDate 2014
url http://irep.iium.edu.my/41424/1/RR-181114-041.pdf
http://irep.iium.edu.my/41424/
http://regres.tesma.org.my/pdf/RR-181114-041.pdf
_version_ 1643612021238792192
score 13.211869

Similar Items