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Toxicity effects of water extracts of Holothuria atra Jaeger in mice

To determine lethal median dose (LD50) and histopathological toxicity of water extract of Holothuria atra (H. atra) in mice. Methods: The behavioral changes, mortality and histopathology examination on liver were assessed in mice 14 d after the administration (i.p.) of H. atra water extract. Seven d...

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Main Authors: Hashim, Ridzwan, Zamli, Zaitunnatakhin, Daud, Jamaluddin
Format: Article
Language:English
Published: Asian Pacific Journal of Tropical Biomedicine 2014
Subjects:
QP Physiology
Online Access:http://irep.iium.edu.my/41145/1/Toxicity_effect_of_water_extracts_of_Holothuria_scabra_Jaeger_in_mice.pdf
http://irep.iium.edu.my/41145/
http://www.ncbi.nlm.nih.gov/pubmed/25183330
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spelling my.iium.irep.411452016-03-04T03:21:26Z http://irep.iium.edu.my/41145/ Toxicity effects of water extracts of Holothuria atra Jaeger in mice Hashim, Ridzwan Zamli, Zaitunnatakhin Daud, Jamaluddin QP Physiology To determine lethal median dose (LD50) and histopathological toxicity of water extract of Holothuria atra (H. atra) in mice. Methods: The behavioral changes, mortality and histopathology examination on liver were assessed in mice 14 d after the administration (i.p.) of H. atra water extract. Seven doses (10, 20, 30, 50, 100, 150 and 200 mg/kg) of H. atra were used. The control group was treated with normal saline. Results: In the acute study in mice, the water extracts of H. atra caused dose-dependent general behavior adverse affects and mortality. The main behavioral sign of toxicity was hypoactivity, noticed immediately after administration of the extract which was more obvious at the higher doses and persisted until death. Mortality increased with increasing doses, the calculated LD50 was 41 mg/kg in mice. The liver toxicity was confirmed by histopathological examination, which indicated the presence of abnormal hepatocytes with a distorted shape and undefined cell lining as well as enlarged nuclei in low doses groups. High doses groups indicated a more prominent distortion of the polyhedral hepatocytes with undefined cell lining, massive cytoplasm, pyknotic, karyorhexis and karyolytic nuclei (necrosis of hepatocytes). Control group showed polyhedral hepatocytes with defined cell lining arranged in cords and normal round nuclei, with granular cytoplasm. Conclusions: Because of the relatively low LD50 value in the acute study in mice, it may be concluded that the H. atra water extract is toxic. Asian Pacific Journal of Tropical Biomedicine 2014 Article REM application/pdf en http://irep.iium.edu.my/41145/1/Toxicity_effect_of_water_extracts_of_Holothuria_scabra_Jaeger_in_mice.pdf Hashim, Ridzwan and Zamli, Zaitunnatakhin and Daud, Jamaluddin (2014) Toxicity effects of water extracts of Holothuria atra Jaeger in mice. Asian Pacific Journal of Tropical Biomedicine, 4 (8). pp. 614-617. ISSN 2221 1691 http://www.ncbi.nlm.nih.gov/pubmed/25183330 10.12980/APJTB.4.201414B154
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QP Physiology
spellingShingle QP Physiology
Hashim, Ridzwan
Zamli, Zaitunnatakhin
Daud, Jamaluddin
Toxicity effects of water extracts of Holothuria atra Jaeger in mice
description To determine lethal median dose (LD50) and histopathological toxicity of water extract of Holothuria atra (H. atra) in mice. Methods: The behavioral changes, mortality and histopathology examination on liver were assessed in mice 14 d after the administration (i.p.) of H. atra water extract. Seven doses (10, 20, 30, 50, 100, 150 and 200 mg/kg) of H. atra were used. The control group was treated with normal saline. Results: In the acute study in mice, the water extracts of H. atra caused dose-dependent general behavior adverse affects and mortality. The main behavioral sign of toxicity was hypoactivity, noticed immediately after administration of the extract which was more obvious at the higher doses and persisted until death. Mortality increased with increasing doses, the calculated LD50 was 41 mg/kg in mice. The liver toxicity was confirmed by histopathological examination, which indicated the presence of abnormal hepatocytes with a distorted shape and undefined cell lining as well as enlarged nuclei in low doses groups. High doses groups indicated a more prominent distortion of the polyhedral hepatocytes with undefined cell lining, massive cytoplasm, pyknotic, karyorhexis and karyolytic nuclei (necrosis of hepatocytes). Control group showed polyhedral hepatocytes with defined cell lining arranged in cords and normal round nuclei, with granular cytoplasm. Conclusions: Because of the relatively low LD50 value in the acute study in mice, it may be concluded that the H. atra water extract is toxic.
format Article
author Hashim, Ridzwan
Zamli, Zaitunnatakhin
Daud, Jamaluddin
author_facet Hashim, Ridzwan
Zamli, Zaitunnatakhin
Daud, Jamaluddin
author_sort Hashim, Ridzwan
title Toxicity effects of water extracts of Holothuria atra Jaeger in mice
title_short Toxicity effects of water extracts of Holothuria atra Jaeger in mice
title_full Toxicity effects of water extracts of Holothuria atra Jaeger in mice
title_fullStr Toxicity effects of water extracts of Holothuria atra Jaeger in mice
title_full_unstemmed Toxicity effects of water extracts of Holothuria atra Jaeger in mice
title_sort toxicity effects of water extracts of holothuria atra jaeger in mice
publisher Asian Pacific Journal of Tropical Biomedicine
publishDate 2014
url http://irep.iium.edu.my/41145/1/Toxicity_effect_of_water_extracts_of_Holothuria_scabra_Jaeger_in_mice.pdf
http://irep.iium.edu.my/41145/
http://www.ncbi.nlm.nih.gov/pubmed/25183330
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score 13.211869

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