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In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves

Lawsonia inermis (henna) is always being explored solely for its scientific components, not for its usage as the Prophetic medicine. Although henna is a popular medicinal plant around the world, only scant toxicity studies have been reported about Malaysia henna and hardly any study that investigat...

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Main Authors: Talik Sisin, Noor Nabilah, Darnis, Deny Susanti
Format: Conference or Workshop Item
Language:English
English
Published: 2014
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/40511/2/POSTER_symposium-2.pdf
http://irep.iium.edu.my/40511/6/in-vivo.pdf
http://irep.iium.edu.my/40511/
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spelling my.iium.irep.405112015-01-06T06:38:50Z http://irep.iium.edu.my/40511/ In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves Talik Sisin, Noor Nabilah Darnis, Deny Susanti Q Science (General) Lawsonia inermis (henna) is always being explored solely for its scientific components, not for its usage as the Prophetic medicine. Although henna is a popular medicinal plant around the world, only scant toxicity studies have been reported about Malaysia henna and hardly any study that investigated histopathological changes of liver and kidneys of mice due to administration of henna leaves extracts. Antiurolithiatic effect of Malaysian henna in-vitro had been proven and in order to continue study the effect in-vivo, toxicity of the henna towards kidneys should be determined first. The objectives of this study are to observe acute oral toxicity effect of the hydroethanolic (75 % ethanol) extract of henna leaves on behavioural and clinical patterns of the mice and to compare the histopathological changes between control and treatment groups of the mice. Leaves were extracted using soxhlet with hydroethanolic solvent. Single oral dose of 2000 mg/kg henna leaves extracts diluted with distilled water were assigned to treatment group of mice while control group was given distilled water only. Observations were done for 14 days and the mice were sacrificed. Selected organs were taken and weighed but only livers and kidneys were taken for histological assessments. Overall, the behavioral and clinical patterns of the mice were observed to be normal. Biostatistically, all selected organs’ means weight between control and treatment groups are not statistically significant (p>0.05). Furthermore, the irregularities found were not very apparent when histopathological examinations were done. Even though there was not death occurred during the experimental period and no gross changes were observed, there were degeneration of connective tissues between the tubules of the kidneys and pyknotic nuclei within hepatocytes. In conclusion, it is scientifically proven that single dose of hydroethanolic extract of L. inermis (henna) leaves can cause toxicity towards liver and kidneys of the mice. 2014 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/40511/2/POSTER_symposium-2.pdf application/pdf en http://irep.iium.edu.my/40511/6/in-vivo.pdf Talik Sisin, Noor Nabilah and Darnis, Deny Susanti (2014) In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves. In: 5th Malaysian Symposium of Biomedical Science, 10th-11th May 2014, UKM Kuala Lumpur Campus, Kuala Lumpur.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Talik Sisin, Noor Nabilah
Darnis, Deny Susanti
In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
description Lawsonia inermis (henna) is always being explored solely for its scientific components, not for its usage as the Prophetic medicine. Although henna is a popular medicinal plant around the world, only scant toxicity studies have been reported about Malaysia henna and hardly any study that investigated histopathological changes of liver and kidneys of mice due to administration of henna leaves extracts. Antiurolithiatic effect of Malaysian henna in-vitro had been proven and in order to continue study the effect in-vivo, toxicity of the henna towards kidneys should be determined first. The objectives of this study are to observe acute oral toxicity effect of the hydroethanolic (75 % ethanol) extract of henna leaves on behavioural and clinical patterns of the mice and to compare the histopathological changes between control and treatment groups of the mice. Leaves were extracted using soxhlet with hydroethanolic solvent. Single oral dose of 2000 mg/kg henna leaves extracts diluted with distilled water were assigned to treatment group of mice while control group was given distilled water only. Observations were done for 14 days and the mice were sacrificed. Selected organs were taken and weighed but only livers and kidneys were taken for histological assessments. Overall, the behavioral and clinical patterns of the mice were observed to be normal. Biostatistically, all selected organs’ means weight between control and treatment groups are not statistically significant (p>0.05). Furthermore, the irregularities found were not very apparent when histopathological examinations were done. Even though there was not death occurred during the experimental period and no gross changes were observed, there were degeneration of connective tissues between the tubules of the kidneys and pyknotic nuclei within hepatocytes. In conclusion, it is scientifically proven that single dose of hydroethanolic extract of L. inermis (henna) leaves can cause toxicity towards liver and kidneys of the mice.
format Conference or Workshop Item
author Talik Sisin, Noor Nabilah
Darnis, Deny Susanti
author_facet Talik Sisin, Noor Nabilah
Darnis, Deny Susanti
author_sort Talik Sisin, Noor Nabilah
title In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
title_short In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
title_full In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
title_fullStr In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
title_full_unstemmed In-vivo toxicity of Prophet's medicinal plant, lawsonia inermis (henna) leaves
title_sort in-vivo toxicity of prophet's medicinal plant, lawsonia inermis (henna) leaves
publishDate 2014
url http://irep.iium.edu.my/40511/2/POSTER_symposium-2.pdf
http://irep.iium.edu.my/40511/6/in-vivo.pdf
http://irep.iium.edu.my/40511/
_version_ 1643611844850483200
score 13.211869

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