Application of response surface methodology for protein enrichment of cassava peel as animal feed by the white-rot fungus Panus tigrinus M609RQY
Response surface methodology (RSM) was employed to optimize the process conditions for production of protein-enriched animal feed from cassava peel by a locally isolated white rot fungus Panus tigrinus. Face-Centered Central Composite Design (FCCCD) with three variables (pH, inoculum size and mois...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Inc.
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/40183/1/Food_hydrocolloids_paper_published_%2C2014.pdf http://irep.iium.edu.my/40183/ http://www.journals.elsevier.com/food-hydrocolloids |
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| Summary: | Response surface methodology (RSM) was employed to optimize the process conditions for production of
protein-enriched animal feed from cassava peel by a locally isolated white rot fungus Panus tigrinus.
Face-Centered Central Composite Design (FCCCD) with three variables (pH, inoculum size and moisture
content) was used to determine the effect of these operational parameters on the protein increase of
cassava peel as animal feed under solid-state fermentation. A significant quadratic model was obtained
for protein increase using this design. Results of the statistical analysis showed that a significant
(P < 0.05) linear effect was obtained for moisture content, while only the interaction effect between
moisture content and inoculum size was significant (P < 0.01). The optimum process combination was
found to be 75% (v/w) of moisture content, a pH of 5.3 and 7% (v/w) inoculum size. A maximum increase
of protein (55.16%) was obtained during 15-day of solid-state fermentation |
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