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Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres

In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres...

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Main Authors: Rahman, Md. Mokhlesur, Mohamed, Awang, Haliza, Katas, Ansary , Rezaul H.
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
QH301 Biology
Online Access:http://irep.iium.edu.my/39998/1/39998.pdf
http://irep.iium.edu.my/39998/
http://www.science.upm.edu.my/isimbiomas2014/
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spelling my.iium.irep.399982018-06-11T03:48:38Z http://irep.iium.edu.my/39998/ Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. QH301 Biology In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins. 2014-10-28 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/39998/1/39998.pdf Rahman, Md. Mokhlesur and Mohamed, Awang and Haliza, Katas and Ansary , Rezaul H. (2014) Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres. In: Malaysia International Biological Symposium 2014 (i-SIMBIOMAS 2014), 28th - 29th October 2014, Putrajaya. http://www.science.upm.edu.my/isimbiomas2014/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
description In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins.
format Conference or Workshop Item
author Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
author_facet Rahman, Md. Mokhlesur
Mohamed, Awang
Haliza, Katas
Ansary , Rezaul H.
author_sort Rahman, Md. Mokhlesur
title Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_short Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_full Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_fullStr Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_full_unstemmed Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
title_sort preparation, characterization and reduction of burst release of bovine serum albumin (bsa) from biodegradable plga microspheres
publishDate 2014
url http://irep.iium.edu.my/39998/1/39998.pdf
http://irep.iium.edu.my/39998/
http://www.science.upm.edu.my/isimbiomas2014/
_version_ 1643616504993808384
score 13.211869

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