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Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines

Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyl...

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Main Authors: Aminuddin, Amnani, Bakhtiar, M. Taher
Format: Conference or Workshop Item
Language:English
English
English
Published: 2014
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf
http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf
http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf
http://irep.iium.edu.my/39555/
http://www.iium.edu.my/ihci
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spelling my.iium.irep.395552015-01-06T10:33:46Z http://irep.iium.edu.my/39555/ Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines Aminuddin, Amnani Bakhtiar, M. Taher RS Pharmacy and materia medica Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyllum D, calanone, and isocordataoblongic acid from Calophyllumsymingtonianum as well as morelloflavone from Garciniaprainiana on MCF 7 human breast cancer cells, RAW 264.7 macrophages and 3T3-L1 pre-adipocytes respectively. The cytotoxicity study on MCF 7 human breast cancer cells was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation respectively. In the cytotoxicity study, inophyllum D was the only compounds that exhibited significant cytotoxic effect against MCF 7 human breast cancer with IC50 of 84 μg/mL. Further, all the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages with inhibition of nitrite concentration as compared to the positive control. Besides, all the compounds in the range of the tested concentrations also produced adipogenic effects on 3T3-L1 pre-adipocytes and this may suggest that they exhibited potential anti-hyperglycemic property which mimicking the insulin action. Thus, this study may provide significant implication in the discovery of the potential of these selected flavonoids as alternative anti-cancer, anti-inflammatory and anti-hyperglycemic drugs. 2014 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf application/pdf en http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf application/pdf en http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf Aminuddin, Amnani and Bakhtiar, M. Taher (2014) Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines. In: International Health Conference IIUM 2014, 3-4 Dec 2014, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/ihci
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Aminuddin, Amnani
Bakhtiar, M. Taher
Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
description Naturally-occurring flavonoids have tremendous potential for producing of new therapeutic agents that provide many benefits to mankind.This study focused on the evaluation of the in vitro cytotoxic, anti-inflammatory and adipocyte differentiation effects of the selected flavonoids which were inophyllum D, calanone, and isocordataoblongic acid from Calophyllumsymingtonianum as well as morelloflavone from Garciniaprainiana on MCF 7 human breast cancer cells, RAW 264.7 macrophages and 3T3-L1 pre-adipocytes respectively. The cytotoxicity study on MCF 7 human breast cancer cells was conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Meanwhile, the study of anti-inflammatory effects in RAW 264.7 macrophages and adipogenic effects on 3T3-L1 pre-adipocytes were conducted through nitrite determination assay and induction of adipocyte differentiation respectively. In the cytotoxicity study, inophyllum D was the only compounds that exhibited significant cytotoxic effect against MCF 7 human breast cancer with IC50 of 84 μg/mL. Further, all the compounds have shown anti-inflammatory effects in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages with inhibition of nitrite concentration as compared to the positive control. Besides, all the compounds in the range of the tested concentrations also produced adipogenic effects on 3T3-L1 pre-adipocytes and this may suggest that they exhibited potential anti-hyperglycemic property which mimicking the insulin action. Thus, this study may provide significant implication in the discovery of the potential of these selected flavonoids as alternative anti-cancer, anti-inflammatory and anti-hyperglycemic drugs.
format Conference or Workshop Item
author Aminuddin, Amnani
Bakhtiar, M. Taher
author_facet Aminuddin, Amnani
Bakhtiar, M. Taher
author_sort Aminuddin, Amnani
title Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_short Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_full Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_fullStr Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_full_unstemmed Cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
title_sort cytotoxic, anti-inflammatory and adipogenic effects of selected flavonoids on cell lines
publishDate 2014
url http://irep.iium.edu.my/39555/2/IHCI_2014_Abstract_Book.pdf
http://irep.iium.edu.my/39555/3/IHCI_2014_Programme_Book.pdf
http://irep.iium.edu.my/39555/8/poster_ihci_amnani-2.pdf
http://irep.iium.edu.my/39555/
http://www.iium.edu.my/ihci
_version_ 1643611659409817600
score 13.211869

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