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Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera

Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictu...

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Main Authors: Mahmod, Safa Senan, Yusof, Faridah, Jami, Mohammed Saedi
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/39224/1/39224.pdf
http://irep.iium.edu.my/39224/
http://iicbe.org/siteadmin/upload/7773C614017.pdf
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spelling my.iium.irep.392242015-04-10T01:45:26Z http://irep.iium.edu.my/39224/ Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi TP248.13 Biotechnology Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity. 2014 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/39224/1/39224.pdf Mahmod, Safa Senan and Yusof, Faridah and Jami, Mohammed Saedi (2014) Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera. In: International Conference on Biological, Chemical and Environmental Sciences (BCES-2014), 14-15 Jun 2014, Penang. http://iicbe.org/siteadmin/upload/7773C614017.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Mahmod, Safa Senan
Yusof, Faridah
Jami, Mohammed Saedi
Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
description Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity.
format Conference or Workshop Item
author Mahmod, Safa Senan
Yusof, Faridah
Jami, Mohammed Saedi
author_facet Mahmod, Safa Senan
Yusof, Faridah
Jami, Mohammed Saedi
author_sort Mahmod, Safa Senan
title Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_short Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_full Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_fullStr Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_full_unstemmed Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
title_sort production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
publishDate 2014
url http://irep.iium.edu.my/39224/1/39224.pdf
http://irep.iium.edu.my/39224/
http://iicbe.org/siteadmin/upload/7773C614017.pdf
_version_ 1643611585949728768
score 13.211869

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