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Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius

A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized i...

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Main Authors: Mohta, Johan Ariff, Yusof, Faridah, Ali, Najala Mahmoud Hag
Format: Article
Language:English
English
Published: AENSI Publications 2014
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/39220/1/P_45.pdf
http://irep.iium.edu.my/39220/4/39220_Screening%20of%20novel%20acidified%20solvents%20for%20maximal_scopus.pdf
http://irep.iium.edu.my/39220/
http://www.aensiweb.com/old/aeb/2014/803-809.pdf
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spelling my.iium.irep.392202020-10-28T04:15:39Z http://irep.iium.edu.my/39220/ Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius Mohta, Johan Ariff Yusof, Faridah Ali, Najala Mahmoud Hag TP248.13 Biotechnology A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized in four solvents at fixed concentration of 90% methanol, 90% ethanol, 90% isopropanol and 90% butanol; each acidified with trifluoroacetic acid (TFA). All samples were extracted at constant solid-solvent ratio (w/v), extraction time and temperature. The solvent extracts were tested on four bacteria in the antimicrobial susceptibility test to evaluate the inhibitory effects. Inhibition zones (mm) were recorded and the range of values was categorized as good, moderate, weak and very weak antibacterial activity. All extracts were found to exhibit significant inhibitory effects on the bacteria (p < 0.001). Isopropanol extract produced significantly (p < 0.05) the largest zone of inhibition in Staphylococcus aureus (10.67 ± 3.06 mm), Escherichia coli (8.67 ± 2.08 mm), Klebsiella pneumonia (8.00 ± 1.00 mm) and Pseudomonas aeroginosa (7.00 ± 2.65 mm) compared to other solvent extracts. The extract was considered a good antibacterial agent against S. aureus, E. coli, and K. pneumonia and as a moderate antibacterial agent against P. aeroginosa. S. aureus was shown to be the most susceptible to isopropanol extract followed by E. coli, K. pneumonia and P. aeroginosa. This finding has a significant implication to improve the extraction procedure over the use of conventional methanolic extraction for new AMP discovery from insect whole body extracts. It appears that acidified isopropanol extracted a maximum amount of AMPs compared to acidified methanol, hence, is selected as the best novel solvent in this study. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39220/1/P_45.pdf application/pdf en http://irep.iium.edu.my/39220/4/39220_Screening%20of%20novel%20acidified%20solvents%20for%20maximal_scopus.pdf Mohta, Johan Ariff and Yusof, Faridah and Ali, Najala Mahmoud Hag (2014) Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius. Advances in Environmental Biology, 8 (3). pp. 803-809. ISSN 1995-0756 E-ISSN 1998-1066 http://www.aensiweb.com/old/aeb/2014/803-809.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Mohta, Johan Ariff
Yusof, Faridah
Ali, Najala Mahmoud Hag
Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
description A solvent screening study using four alcohols with decreasing polarity to select novel acidified extraction solvents for maximal antimicrobial peptide (AMPs) recovery from the whole body extracts of supermealworm Zophobas morio larvae was performed. 20 grams of final instar larvae were homogenized in four solvents at fixed concentration of 90% methanol, 90% ethanol, 90% isopropanol and 90% butanol; each acidified with trifluoroacetic acid (TFA). All samples were extracted at constant solid-solvent ratio (w/v), extraction time and temperature. The solvent extracts were tested on four bacteria in the antimicrobial susceptibility test to evaluate the inhibitory effects. Inhibition zones (mm) were recorded and the range of values was categorized as good, moderate, weak and very weak antibacterial activity. All extracts were found to exhibit significant inhibitory effects on the bacteria (p < 0.001). Isopropanol extract produced significantly (p < 0.05) the largest zone of inhibition in Staphylococcus aureus (10.67 ± 3.06 mm), Escherichia coli (8.67 ± 2.08 mm), Klebsiella pneumonia (8.00 ± 1.00 mm) and Pseudomonas aeroginosa (7.00 ± 2.65 mm) compared to other solvent extracts. The extract was considered a good antibacterial agent against S. aureus, E. coli, and K. pneumonia and as a moderate antibacterial agent against P. aeroginosa. S. aureus was shown to be the most susceptible to isopropanol extract followed by E. coli, K. pneumonia and P. aeroginosa. This finding has a significant implication to improve the extraction procedure over the use of conventional methanolic extraction for new AMP discovery from insect whole body extracts. It appears that acidified isopropanol extracted a maximum amount of AMPs compared to acidified methanol, hence, is selected as the best novel solvent in this study.
format Article
author Mohta, Johan Ariff
Yusof, Faridah
Ali, Najala Mahmoud Hag
author_facet Mohta, Johan Ariff
Yusof, Faridah
Ali, Najala Mahmoud Hag
author_sort Mohta, Johan Ariff
title Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_short Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_full Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_fullStr Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_full_unstemmed Screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
title_sort screening of novel acidified solvents for maximal antimicrobial peptide extraction from zophobas morio fabricius
publisher AENSI Publications
publishDate 2014
url http://irep.iium.edu.my/39220/1/P_45.pdf
http://irep.iium.edu.my/39220/4/39220_Screening%20of%20novel%20acidified%20solvents%20for%20maximal_scopus.pdf
http://irep.iium.edu.my/39220/
http://www.aensiweb.com/old/aeb/2014/803-809.pdf
_version_ 1683230279351664640
score 13.211869

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