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Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria

Non-polar (dichloromethane) and polar (MeOH and aqueous) extracts of Andrographis paniculata(whole plant) were evaluated for in vitro antibacterial activity against 10 skin disease causing bacterial strains (6 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Staphylococ...

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Main Authors: Ahmed, Qamar Uddin, Sule, Abubakar, Abd. Samah, Othman, Omar, Muhammad Nor
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
RM Therapeutics. Pharmacology
Online Access:http://irep.iium.edu.my/3920/1/Bacteriostatic_and_bactericidal_activities_of.pdf
http://irep.iium.edu.my/3920/
http://www.academicjournals.org/jmpr/
http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/4Jan/Sule%20et%20al.htm
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spelling my.iium.irep.39202012-02-01T03:15:49Z http://irep.iium.edu.my/3920/ Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria Ahmed, Qamar Uddin Sule, Abubakar Abd. Samah, Othman Omar, Muhammad Nor RM Therapeutics. Pharmacology Non-polar (dichloromethane) and polar (MeOH and aqueous) extracts of Andrographis paniculata(whole plant) were evaluated for in vitro antibacterial activity against 10 skin disease causing bacterial strains (6 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Micrococcus luteus) and 4 gram negative strains (Proteus mirabilis, Proteus vulgaris, Neisseria meningitis, Pseudomonas aeruginosa) using disc diffusion method at three different concentrations; 1000, 500 and 250 μg/disc respectively. The extracts showed significant antibacterial activities against both Gram-positive and Gram-negative bacterial strains tested. Highest significant antibacterial activity was exerted by the aqueous extract against M. luteus at 1000 μg/disc and the least activity was exhibited by the DCM extract against N. meningitis at 250 μg/disc. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) observed were between 150 to 300 μg/ml and 250 to 400 μg/ml respectively, depending on microorganism and the nature of various extracts. Time-kill experiments indicated that A. paniculata extracts have bactericidal characteristic against most of the Gram positive bacteria and bacteriostatic activity against both Gram negative and Gram positive bacteria. These results candidly suggest the presence of promising antibacterial substances in the polar as well as non-polar extracts which could be the source of potential phytomedicine for the treatment of skin infections caused by the pathogenic bacterial strains. Our findings explicitly support its traditional claims and form a strong basis for further sincere efforts to explore A. paniculata’s antibacterial potential to treat skin frailties efficaciously. Academic Journals 2011-01-04 Article REM application/pdf en cc_by_nc http://irep.iium.edu.my/3920/1/Bacteriostatic_and_bactericidal_activities_of.pdf Ahmed, Qamar Uddin and Sule, Abubakar and Abd. Samah, Othman and Omar, Muhammad Nor (2011) Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria. Journal of Medicinal Plants Research, 5 (1). pp. 7-14. ISSN 1996-0875 http://www.academicjournals.org/jmpr/ http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/4Jan/Sule%20et%20al.htm
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Ahmed, Qamar Uddin
Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
description Non-polar (dichloromethane) and polar (MeOH and aqueous) extracts of Andrographis paniculata(whole plant) were evaluated for in vitro antibacterial activity against 10 skin disease causing bacterial strains (6 gram positive strains; Staphylococcus saprophyticus, Staphylococcus epidermis, Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Micrococcus luteus) and 4 gram negative strains (Proteus mirabilis, Proteus vulgaris, Neisseria meningitis, Pseudomonas aeruginosa) using disc diffusion method at three different concentrations; 1000, 500 and 250 μg/disc respectively. The extracts showed significant antibacterial activities against both Gram-positive and Gram-negative bacterial strains tested. Highest significant antibacterial activity was exerted by the aqueous extract against M. luteus at 1000 μg/disc and the least activity was exhibited by the DCM extract against N. meningitis at 250 μg/disc. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) observed were between 150 to 300 μg/ml and 250 to 400 μg/ml respectively, depending on microorganism and the nature of various extracts. Time-kill experiments indicated that A. paniculata extracts have bactericidal characteristic against most of the Gram positive bacteria and bacteriostatic activity against both Gram negative and Gram positive bacteria. These results candidly suggest the presence of promising antibacterial substances in the polar as well as non-polar extracts which could be the source of potential phytomedicine for the treatment of skin infections caused by the pathogenic bacterial strains. Our findings explicitly support its traditional claims and form a strong basis for further sincere efforts to explore A. paniculata’s antibacterial potential to treat skin frailties efficaciously.
format Article
author Ahmed, Qamar Uddin
Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
author_facet Ahmed, Qamar Uddin
Sule, Abubakar
Abd. Samah, Othman
Omar, Muhammad Nor
author_sort Ahmed, Qamar Uddin
title Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
title_short Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
title_full Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
title_fullStr Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
title_full_unstemmed Bacteriostatic and bactericidal activities of Andrographis paniculata extracts on skin disease causing pathogenic bacteria
title_sort bacteriostatic and bactericidal activities of andrographis paniculata extracts on skin disease causing pathogenic bacteria
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/3920/1/Bacteriostatic_and_bactericidal_activities_of.pdf
http://irep.iium.edu.my/3920/
http://www.academicjournals.org/jmpr/
http://www.academicjournals.org/jmpr/abstracts/abstracts/abstracts2011/4Jan/Sule%20et%20al.htm
_version_ 1643605234119868416
score 13.211869

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