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Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design

Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Pl...

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Main Authors: Alam, Md Zahangir, Zainan, Nur Hidayah, Al-Khatib, Ma'an Fahmi Rashid
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2014
Subjects:
TP315 Fuel
Online Access:http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf
http://irep.iium.edu.my/38271/
http://www.microbiologyjournal.org/
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spelling my.iium.irep.382712024-05-02T04:35:47Z http://irep.iium.edu.my/38271/ Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design Alam, Md Zahangir Zainan, Nur Hidayah Al-Khatib, Ma'an Fahmi Rashid TP315 Fuel Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Plackett-Burman (PB) design, one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD). Among the parameters tested, pH, temperature, inoculum size, potassium dihydrogen phosphate (KH2PO4), magnesium sulphate heptahydrate (MgSO4.7H2O) and peptone showed positive effects while yeast extract, malt extract, potassium chloride, urea and agitation were influencing the production negatively. The three parameters chosen for determination of the optimum values by response surface methodology (RSM) based on the FCCCD were pH, KH2PO4 and agitation. Although agitation showed negative effects it was considered in FCCCD due to the mixing effect of fermnetation. The validity of the model was verified and the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum bioethanol production of 7.4 g/l. The yield of bioethanol was determined based on the reducing sugar (16.85 g/l) obtained from the EFB. Journal of Pure and Applied Microbiology 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf Alam, Md Zahangir and Zainan, Nur Hidayah and Al-Khatib, Ma'an Fahmi Rashid (2014) Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 731-740. ISSN 0973-7510 http://www.microbiologyjournal.org/
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP315 Fuel
spellingShingle TP315 Fuel
Alam, Md Zahangir
Zainan, Nur Hidayah
Al-Khatib, Ma'an Fahmi Rashid
Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
description Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Plackett-Burman (PB) design, one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD). Among the parameters tested, pH, temperature, inoculum size, potassium dihydrogen phosphate (KH2PO4), magnesium sulphate heptahydrate (MgSO4.7H2O) and peptone showed positive effects while yeast extract, malt extract, potassium chloride, urea and agitation were influencing the production negatively. The three parameters chosen for determination of the optimum values by response surface methodology (RSM) based on the FCCCD were pH, KH2PO4 and agitation. Although agitation showed negative effects it was considered in FCCCD due to the mixing effect of fermnetation. The validity of the model was verified and the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum bioethanol production of 7.4 g/l. The yield of bioethanol was determined based on the reducing sugar (16.85 g/l) obtained from the EFB.
format Article
author Alam, Md Zahangir
Zainan, Nur Hidayah
Al-Khatib, Ma'an Fahmi Rashid
author_facet Alam, Md Zahangir
Zainan, Nur Hidayah
Al-Khatib, Ma'an Fahmi Rashid
author_sort Alam, Md Zahangir
title Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
title_short Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
title_full Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
title_fullStr Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
title_full_unstemmed Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
title_sort optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
publisher Journal of Pure and Applied Microbiology
publishDate 2014
url http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf
http://irep.iium.edu.my/38271/
http://www.microbiologyjournal.org/
_version_ 1800081736889008128
score 13.211869

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