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The growth study of vero cells in different type of microcarrier

The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of...

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Main Authors: Ahmad Nor, Yusilawati, Sulong, Nurul Hafizah, Mel, Maizirwan, Mohd. Salleh, Hamzah, Sopyan, Iis
Format: Article
Language:English
Published: Scientific Research 2010
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf
http://irep.iium.edu.my/3721/
http://www.scirp.org/journal/PaperInformation.aspx?paperID=3286
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spelling my.iium.irep.37212021-07-06T01:43:17Z http://irep.iium.edu.my/3721/ The growth study of vero cells in different type of microcarrier Ahmad Nor, Yusilawati Sulong, Nurul Hafizah Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis TP248.13 Biotechnology The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3, Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex- 3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor. Scientific Research 2010-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf Ahmad Nor, Yusilawati and Sulong, Nurul Hafizah and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis (2010) The growth study of vero cells in different type of microcarrier. Materials Sciences and Applications, 1 (5). pp. 261-266. ISSN 2153-1188 (O), 2153-117X (P) http://www.scirp.org/journal/PaperInformation.aspx?paperID=3286 doi:10.4236/msa.2010.15038
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Ahmad Nor, Yusilawati
Sulong, Nurul Hafizah
Mel, Maizirwan
Mohd. Salleh, Hamzah
Sopyan, Iis
The growth study of vero cells in different type of microcarrier
description The fact of microcarrier (MC) culture introduces new possibilities and makes possible the practical high-yield culture of anchorage-dependent cells has generated a considerable focus in this study. The objective of this research was to study the comparison of Vero cell growth on different types of commercial microcarriers; Cytodex-1, Cytodex-3, Hillex® II and Plastic Plus in spinner vessel and two liters bioreactor cultured for 96 hours. Biological performance of the microcarrier in RPMI media showed the preference of Vero cell grew on Cytodex 3 microcarriers with highest maximum viable cell number (2.4 × 105 cells/ml) followed by Cytodex 1, Hillex and Plustic Plus. Vero cell on Cytodex- 3 data in spinner flask was compared in bioreactor and result showed higher viable cell number in biorector. Thus, this dextran-crosslink gelatin microcarrier (Cytodex 3) provided the best surface for cell attachment and fast proliferation. At the end of this cell growth improvement will be used for virus transfection producing a vaccine in bioreactor.
format Article
author Ahmad Nor, Yusilawati
Sulong, Nurul Hafizah
Mel, Maizirwan
Mohd. Salleh, Hamzah
Sopyan, Iis
author_facet Ahmad Nor, Yusilawati
Sulong, Nurul Hafizah
Mel, Maizirwan
Mohd. Salleh, Hamzah
Sopyan, Iis
author_sort Ahmad Nor, Yusilawati
title The growth study of vero cells in different type of microcarrier
title_short The growth study of vero cells in different type of microcarrier
title_full The growth study of vero cells in different type of microcarrier
title_fullStr The growth study of vero cells in different type of microcarrier
title_full_unstemmed The growth study of vero cells in different type of microcarrier
title_sort growth study of vero cells in different type of microcarrier
publisher Scientific Research
publishDate 2010
url http://irep.iium.edu.my/3721/1/MSA-Microcarrier_2010.pdf
http://irep.iium.edu.my/3721/
http://www.scirp.org/journal/PaperInformation.aspx?paperID=3286
_version_ 1705056443149844480
score 13.211869

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