Cover Image

Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia

This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mert...

Full description

Saved in:
Bibliographic Details
Main Authors: Lukman, Ahmad Lutfi, Nordin, Noor Faizul Hadry, Kamarudin, Kamarul Rahim
Format: Article
Language:English
English
English
Published: Penerbit UKM 2014
Subjects:
QR Microbiology
Online Access:http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf
http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf
http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf
http://irep.iium.edu.my/37173/
http://www.ukm.my/jsm/malay_journals/jilid43bil7_2014/KandunganJilid43Bil7_2014.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.37173
record_format dspace
spelling my.iium.irep.37173 http://irep.iium.edu.my/37173/ Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia Lukman, Ahmad Lutfi Nordin, Noor Faizul Hadry Kamarudin, Kamarul Rahim QR Microbiology This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species. Penerbit UKM 2014-07-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf application/pdf en http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf Lukman, Ahmad Lutfi and Nordin, Noor Faizul Hadry and Kamarudin, Kamarul Rahim (2014) Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia. Sains Malaysiana, 43 (7). pp. 1013-1021. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid43bil7_2014/KandunganJilid43Bil7_2014.html
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
English
topic QR Microbiology
spellingShingle QR Microbiology
Lukman, Ahmad Lutfi
Nordin, Noor Faizul Hadry
Kamarudin, Kamarul Rahim
Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
description This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species.
format Article
author Lukman, Ahmad Lutfi
Nordin, Noor Faizul Hadry
Kamarudin, Kamarul Rahim
author_facet Lukman, Ahmad Lutfi
Nordin, Noor Faizul Hadry
Kamarudin, Kamarul Rahim
author_sort Lukman, Ahmad Lutfi
title Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_short Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_full Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_fullStr Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_full_unstemmed Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from Malaysian waters = Populasi Mikrob dalam Cecair Selom Stichopus chloronotus dan Holothuria (Mertensiothuria) leucospilota dari Perairan Malaysia
title_sort microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from malaysian waters = populasi mikrob dalam cecair selom stichopus chloronotus dan holothuria (mertensiothuria) leucospilota dari perairan malaysia
publisher Penerbit UKM
publishDate 2014
url http://irep.iium.edu.my/37173/1/2014_Sains_Malaysiana_Ahmad_Lutfi_et_al.pdf
http://irep.iium.edu.my/37173/4/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_SCOPUS.pdf
http://irep.iium.edu.my/37173/5/37173_Microbial%20population%20in%20the%20coelomic%20fluid%20of%20stichopus%20chloronotus%20and%20holothuria_WoS.pdf
http://irep.iium.edu.my/37173/
http://www.ukm.my/jsm/malay_journals/jilid43bil7_2014/KandunganJilid43Bil7_2014.html
_version_ 1643616643726704640
score 13.211869

Similar Items