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Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage

ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains u...

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Main Authors: Lestari, Widya, Arief Ichwan, Solachuddin Jauhari
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
RK Dentistry
Online Access:http://irep.iium.edu.my/36693/1/36693.pdf
http://irep.iium.edu.my/36693/
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spelling my.iium.irep.366932015-05-12T03:06:54Z http://irep.iium.edu.my/36693/ Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage Lestari, Widya Arief Ichwan, Solachuddin Jauhari RK Dentistry ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains unclear. Here, ‘we show that ARID3A cooperates with p53 to transcriptionally activate p21WAF1, a p53-target gene important for cell-cycle arrest. ARID3A bound to its binding sites in the p21WAF1 promoter in vivo and in vitro, and induced p21WAF1 transcription in U2OS cells expressing wild-type p53 but not Saos-2 cells lacking p53. Co-expression of ARID3A with p53 cooperates to activate p21WAF1 transcription and the stably transfected p21WAF1 promoter. Mutation of the ARID3A binding sites reduced the p21WAF1 promoter activity, and siRNA-based ARID3A knockdown suppressed transcription of p21WAF1 but not proapoptotic NOXA and PUMA in response to DNA damage. Furthermore, p53 knockdown decreased ARID3A transcription, and, conversely, ARID3A overexpression or knockdown resulted in an increase or decrease in p53 stability, respectively. These results indicate cooperative and interdependent roles for ARID3A and p53 in transcriptional activation of p21WAF1 in response to DNA damage. 2013 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/36693/1/36693.pdf Lestari, Widya and Arief Ichwan, Solachuddin Jauhari (2013) Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2013, 19-20th February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RK Dentistry
spellingShingle RK Dentistry
Lestari, Widya
Arief Ichwan, Solachuddin Jauhari
Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
description ARID3A/DRIL1/Bright is a family member of AT rich interaction domain (ARID) DNA-binding proteins that involve in diverse biological processes. We have reported that p53 activates ARID3A transcription, and ARID3A overexpression induced G1 arrest. However, a role of ARID3A in the p53 pathway remains unclear. Here, ‘we show that ARID3A cooperates with p53 to transcriptionally activate p21WAF1, a p53-target gene important for cell-cycle arrest. ARID3A bound to its binding sites in the p21WAF1 promoter in vivo and in vitro, and induced p21WAF1 transcription in U2OS cells expressing wild-type p53 but not Saos-2 cells lacking p53. Co-expression of ARID3A with p53 cooperates to activate p21WAF1 transcription and the stably transfected p21WAF1 promoter. Mutation of the ARID3A binding sites reduced the p21WAF1 promoter activity, and siRNA-based ARID3A knockdown suppressed transcription of p21WAF1 but not proapoptotic NOXA and PUMA in response to DNA damage. Furthermore, p53 knockdown decreased ARID3A transcription, and, conversely, ARID3A overexpression or knockdown resulted in an increase or decrease in p53 stability, respectively. These results indicate cooperative and interdependent roles for ARID3A and p53 in transcriptional activation of p21WAF1 in response to DNA damage.
format Conference or Workshop Item
author Lestari, Widya
Arief Ichwan, Solachuddin Jauhari
author_facet Lestari, Widya
Arief Ichwan, Solachuddin Jauhari
author_sort Lestari, Widya
title Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_short Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_full Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_fullStr Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_full_unstemmed Cooperation between ARID3A and p53 in the transcriptional activation of p21WAF1 in response to DNA damage
title_sort cooperation between arid3a and p53 in the transcriptional activation of p21waf1 in response to dna damage
publishDate 2013
url http://irep.iium.edu.my/36693/1/36693.pdf
http://irep.iium.edu.my/36693/
_version_ 1643611044622368768
score 13.211869

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