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A lab-on-chip cell-based biosensor for label-free sensing of water toxicants

This paper presents a lab-on-chip biosensor containing an enclosed fluidic cell culturing well seeded with live cells for rapid screening of toxicants in drinking water. The sensor is based on the innovative placement of the working electrode for the electrical cell–substrate impedance sensing (EC...

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Main Author: Nordin, Anis Nurashikin
Format: Article
Language:English
Published: Royal Society of Chemistry 2014
Subjects:
T Technology (General)
Online Access:http://irep.iium.edu.my/36648/1/C3LC51085A%281%29.pdf
http://irep.iium.edu.my/36648/
http://pubs.rsc.org/EN/content/articlehtml/2013/lc/c3lc51085a
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spelling my.iium.irep.366482014-05-19T00:29:20Z http://irep.iium.edu.my/36648/ A lab-on-chip cell-based biosensor for label-free sensing of water toxicants Nordin, Anis Nurashikin T Technology (General) This paper presents a lab-on-chip biosensor containing an enclosed fluidic cell culturing well seeded with live cells for rapid screening of toxicants in drinking water. The sensor is based on the innovative placement of the working electrode for the electrical cell–substrate impedance sensing (ECIS) technique as the top electrode of a quartz crystal microbalance (QCM) resonator. Cell damage induced by toxic water will cause a decrease in impedance, as well as an increase in the resonant frequency. For water toxicity tests, the biosensor's unique capabilities of performing two complementary measurements simultaneously (impedance and mass-sensing) will increase the accuracy of detection while decreasing the false-positive rate. Bovine aortic endothelial cells (BAECs) were used as toxicity sensing cells. The effects of the toxicants, ammonia, nicotine and aldicarb, on cells were monitored with both the QCM and the ECIS technique. The lab-on-chip was demonstrated to be sensitive to low concentrations of toxicants. The responses of BAECs to toxic samples occurred during the initial 5 to 20 minutes depending on the type of chemical and concentrations. Testing the multiparameter biosensor with aldicarb also demonstrated the hypothesis that using two different sensors to monitor the same cell monolayer provides cross validation and increases the accuracy of detection. For low concentrations of aldicarb, the variations in impedance measurements are insignificant in comparison with the shifts of resonant frequency monitored using the QCM resonator. A highly linear correlation between signal shifts and chemical concentrations was demonstrated for each toxicant. Royal Society of Chemistry 2014 Article REM application/pdf en http://irep.iium.edu.my/36648/1/C3LC51085A%281%29.pdf Nordin, Anis Nurashikin (2014) A lab-on-chip cell-based biosensor for label-free sensing of water toxicants. Lab on a Chip (14). pp. 1270-1280. http://pubs.rsc.org/EN/content/articlehtml/2013/lc/c3lc51085a 10.1039/c3lc51085a
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Nordin, Anis Nurashikin
A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
description This paper presents a lab-on-chip biosensor containing an enclosed fluidic cell culturing well seeded with live cells for rapid screening of toxicants in drinking water. The sensor is based on the innovative placement of the working electrode for the electrical cell–substrate impedance sensing (ECIS) technique as the top electrode of a quartz crystal microbalance (QCM) resonator. Cell damage induced by toxic water will cause a decrease in impedance, as well as an increase in the resonant frequency. For water toxicity tests, the biosensor's unique capabilities of performing two complementary measurements simultaneously (impedance and mass-sensing) will increase the accuracy of detection while decreasing the false-positive rate. Bovine aortic endothelial cells (BAECs) were used as toxicity sensing cells. The effects of the toxicants, ammonia, nicotine and aldicarb, on cells were monitored with both the QCM and the ECIS technique. The lab-on-chip was demonstrated to be sensitive to low concentrations of toxicants. The responses of BAECs to toxic samples occurred during the initial 5 to 20 minutes depending on the type of chemical and concentrations. Testing the multiparameter biosensor with aldicarb also demonstrated the hypothesis that using two different sensors to monitor the same cell monolayer provides cross validation and increases the accuracy of detection. For low concentrations of aldicarb, the variations in impedance measurements are insignificant in comparison with the shifts of resonant frequency monitored using the QCM resonator. A highly linear correlation between signal shifts and chemical concentrations was demonstrated for each toxicant.
format Article
author Nordin, Anis Nurashikin
author_facet Nordin, Anis Nurashikin
author_sort Nordin, Anis Nurashikin
title A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
title_short A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
title_full A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
title_fullStr A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
title_full_unstemmed A lab-on-chip cell-based biosensor for label-free sensing of water toxicants
title_sort lab-on-chip cell-based biosensor for label-free sensing of water toxicants
publisher Royal Society of Chemistry
publishDate 2014
url http://irep.iium.edu.my/36648/1/C3LC51085A%281%29.pdf
http://irep.iium.edu.my/36648/
http://pubs.rsc.org/EN/content/articlehtml/2013/lc/c3lc51085a
_version_ 1643611034035945472
score 13.211869

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