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Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis

Cetirizine is a second generation anti-histamin H1-receptor antagonist, used to treat cold or allergy. This drug is a white, crystalline powder, freely soluble in water, practically insoluble in acetone and in methylene chloride. Cetirizine is a non-sedating antihistamin because it crossed blood...

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Main Authors: Amiruddin, Noor Syafawati, Helal Uddin, A.B.M., Mohamad, Huda Jamilah, Al-Aamaa, Mohamed
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
QD Chemistry
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/34954/1/Evaluation_of_different_extraction_method_for_the_isolation_of_Cetirizine_from_......pdf
http://irep.iium.edu.my/34954/
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spelling my.iium.irep.349542022-11-07T04:00:24Z http://irep.iium.edu.my/34954/ Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis Amiruddin, Noor Syafawati Helal Uddin, A.B.M. Mohamad, Huda Jamilah Al-Aamaa, Mohamed QD Chemistry RS Pharmacy and materia medica Cetirizine is a second generation anti-histamin H1-receptor antagonist, used to treat cold or allergy. This drug is a white, crystalline powder, freely soluble in water, practically insoluble in acetone and in methylene chloride. Cetirizine is a non-sedating antihistamin because it crossed blood brain barrier slightly,reduces sedative side effect. In allergic inflammatory process, this drug influences the activities of eosinophils, neutrophils and platelets.It inhibits eosinophils in allergen-induced type I skin reaction and eosinophil chemotaxis in vitro by reducing the expression of ICAM-1and soluble ICAM-1in epithelial cells.Malaysian government has implemented bioequivalence study for the registration of generic products and our study is in line with the regulatory requirement. Besides so far there is no report of such study on cetirizine in Malaysia.The study focused on the preliminary chromatographic detection and determination of cetirizine in human plasma. Different sample extraction process were evaluated namely protein precipitation and liquid-liquid extraction. In protein precipitation, two solvents were used; acetonitrile and chloroform and for liquid� liquid extraction, dichloromethane and ethyl acetate with the various buffers were used. It was found protein precipitation using acetonitrile showed a better recovery (86%)compared to chloroform. For the liquid liquid extraction using dichloromethane the recovery was 80% while with the addition of buffer it did not show a good result. Liquid liquid extraction using dichloromethane with addition of sodium chloride showed 52% recovery.High Performance Liquid Chromatography(HPLC)was used for the analysis of the spiked plasma samples.The column used was aC18 column and mobile phase was combination of trietylamine and acetonitrile with the ratio of 65:35.Elution of cetirizine was detected with a UV-Vis detector at 232nm wavelength. 2013 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/34954/1/Evaluation_of_different_extraction_method_for_the_isolation_of_Cetirizine_from_......pdf Amiruddin, Noor Syafawati and Helal Uddin, A.B.M. and Mohamad, Huda Jamilah and Al-Aamaa, Mohamed (2013) Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis. In: 2nd International Postgraduate Conference on Pharmaceutical Sciences 2013 (iPoPS 2013), 4-5 September 2013, Shah Alam, Selangor.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
RS Pharmacy and materia medica
spellingShingle QD Chemistry
RS Pharmacy and materia medica
Amiruddin, Noor Syafawati
Helal Uddin, A.B.M.
Mohamad, Huda Jamilah
Al-Aamaa, Mohamed
Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
description Cetirizine is a second generation anti-histamin H1-receptor antagonist, used to treat cold or allergy. This drug is a white, crystalline powder, freely soluble in water, practically insoluble in acetone and in methylene chloride. Cetirizine is a non-sedating antihistamin because it crossed blood brain barrier slightly,reduces sedative side effect. In allergic inflammatory process, this drug influences the activities of eosinophils, neutrophils and platelets.It inhibits eosinophils in allergen-induced type I skin reaction and eosinophil chemotaxis in vitro by reducing the expression of ICAM-1and soluble ICAM-1in epithelial cells.Malaysian government has implemented bioequivalence study for the registration of generic products and our study is in line with the regulatory requirement. Besides so far there is no report of such study on cetirizine in Malaysia.The study focused on the preliminary chromatographic detection and determination of cetirizine in human plasma. Different sample extraction process were evaluated namely protein precipitation and liquid-liquid extraction. In protein precipitation, two solvents were used; acetonitrile and chloroform and for liquid� liquid extraction, dichloromethane and ethyl acetate with the various buffers were used. It was found protein precipitation using acetonitrile showed a better recovery (86%)compared to chloroform. For the liquid liquid extraction using dichloromethane the recovery was 80% while with the addition of buffer it did not show a good result. Liquid liquid extraction using dichloromethane with addition of sodium chloride showed 52% recovery.High Performance Liquid Chromatography(HPLC)was used for the analysis of the spiked plasma samples.The column used was aC18 column and mobile phase was combination of trietylamine and acetonitrile with the ratio of 65:35.Elution of cetirizine was detected with a UV-Vis detector at 232nm wavelength.
format Conference or Workshop Item
author Amiruddin, Noor Syafawati
Helal Uddin, A.B.M.
Mohamad, Huda Jamilah
Al-Aamaa, Mohamed
author_facet Amiruddin, Noor Syafawati
Helal Uddin, A.B.M.
Mohamad, Huda Jamilah
Al-Aamaa, Mohamed
author_sort Amiruddin, Noor Syafawati
title Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
title_short Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
title_full Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
title_fullStr Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
title_full_unstemmed Evaluation of different extraction method for the isolation of cetirizine from human plasma for HPLC analysis
title_sort evaluation of different extraction method for the isolation of cetirizine from human plasma for hplc analysis
publishDate 2013
url http://irep.iium.edu.my/34954/1/Evaluation_of_different_extraction_method_for_the_isolation_of_Cetirizine_from_......pdf
http://irep.iium.edu.my/34954/
_version_ 1751535883940528128
score 13.211869

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