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In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load

Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV pati...

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Main Authors: Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, Abdullah, Nasuruddin
Format: Conference or Workshop Item
Language:English
Published: 2013
Subjects:
QR355 Virology
Online Access:http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf
http://irep.iium.edu.my/34879/
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spelling my.iium.irep.348792014-01-28T04:48:55Z http://irep.iium.edu.my/34879/ In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin QR355 Virology Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. 2013 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah, Nasuruddin (2013) In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load. In: IIUM Research, Invention and Innovation Exhibition 2013, 19 - 20 February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR355 Virology
spellingShingle QR355 Virology
Hamzah, Hairul Aini
Mustafa Mahmoud, Mohammed Imad Al-Deen
Abdullah, Nasuruddin
In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
description Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum.
format Conference or Workshop Item
author Hamzah, Hairul Aini
Mustafa Mahmoud, Mohammed Imad Al-Deen
Abdullah, Nasuruddin
author_facet Hamzah, Hairul Aini
Mustafa Mahmoud, Mohammed Imad Al-Deen
Abdullah, Nasuruddin
author_sort Hamzah, Hairul Aini
title In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
title_short In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
title_full In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
title_fullStr In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
title_full_unstemmed In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
title_sort in-house semi-nested reverse transcription polymerase chain reaction for the detection of hcv-rna in serum with low viral load
publishDate 2013
url http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf
http://irep.iium.edu.my/34879/
_version_ 1643610696980627456
score 13.211869

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