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Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions

A study was conducted to determine the total content of carotenoids, in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant aeration (4.5 L min-1) at 8 pH. Salinity of the water was maintained betw...

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Main Authors: Khan Chowdhury, Ahmed Jalal, Shamsudin, A.A, Rahman, M.F, Nurzatul , N.Z, Rozihan, M
Format: Article
Language:English
Published: Asian Network for Scientific Information 2013
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/34073/1/10-17.pdf
http://irep.iium.edu.my/34073/
http://scialert.net/jindex.php?issn=1727-3048
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spelling my.iium.irep.340732017-12-08T02:30:43Z http://irep.iium.edu.my/34073/ Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions Khan Chowdhury, Ahmed Jalal Shamsudin, A.A Rahman, M.F Nurzatul , N.Z Rozihan, M Q Science (General) A study was conducted to determine the total content of carotenoids, in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant aeration (4.5 L min-1) at 8 pH. Salinity of the water was maintained between 20-24 g L-1 and temperature range was 20-24°C. The culture of Isochrysis sp. showed a short lag phase lasting about 24 h followed by exponential phase starting from day 2 until day 10. In day 4, specific growth rate (u.) for Isochrysis sp. was 1.6059 day-1, the highest rate compared to other day. Stress parameter (NO- 3 starvation) was introduced into the mass culture on day 4 by eliminating NO- 3~ in f/2 media. There was considerable declining (p<0.05) in cell count was observed in the absence of nitrogen proving the dependency of carotenoid production over the nitrogen limitation which probably due to the main role played by the nitrogen in various metabolic activities of the cell. The highest total carotenoid per cell was recorded at day 10 with 0.001932347 mg mL-1, whereas the lowest total carotenoid content per cell was observed at day 4 with 0.000100649 mg mL-1. The finding reveals that it is best to harvest the carotenoid on day 10, when the maximum carotenoid can be obtained. © 2013 Asian Network for Scientific Information. Asian Network for Scientific Information 2013 Article REM application/pdf en http://irep.iium.edu.my/34073/1/10-17.pdf Khan Chowdhury, Ahmed Jalal and Shamsudin, A.A and Rahman, M.F and Nurzatul , N.Z and Rozihan, M (2013) Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions. Journal of Biological Sciences, 13 (1). pp. 10-17. ISSN 17273048 (P), 1812-5719 (O) http://scialert.net/jindex.php?issn=1727-3048
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Khan Chowdhury, Ahmed Jalal
Shamsudin, A.A
Rahman, M.F
Nurzatul , N.Z
Rozihan, M
Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
description A study was conducted to determine the total content of carotenoids, in Isochrysis sp. under stress condition (nitrogen starvation). Isochrysis sp. was cultured in f/2 medium at optimum light intensity (1200 Lux) with constant aeration (4.5 L min-1) at 8 pH. Salinity of the water was maintained between 20-24 g L-1 and temperature range was 20-24°C. The culture of Isochrysis sp. showed a short lag phase lasting about 24 h followed by exponential phase starting from day 2 until day 10. In day 4, specific growth rate (u.) for Isochrysis sp. was 1.6059 day-1, the highest rate compared to other day. Stress parameter (NO- 3 starvation) was introduced into the mass culture on day 4 by eliminating NO- 3~ in f/2 media. There was considerable declining (p<0.05) in cell count was observed in the absence of nitrogen proving the dependency of carotenoid production over the nitrogen limitation which probably due to the main role played by the nitrogen in various metabolic activities of the cell. The highest total carotenoid per cell was recorded at day 10 with 0.001932347 mg mL-1, whereas the lowest total carotenoid content per cell was observed at day 4 with 0.000100649 mg mL-1. The finding reveals that it is best to harvest the carotenoid on day 10, when the maximum carotenoid can be obtained. © 2013 Asian Network for Scientific Information.
format Article
author Khan Chowdhury, Ahmed Jalal
Shamsudin, A.A
Rahman, M.F
Nurzatul , N.Z
Rozihan, M
author_facet Khan Chowdhury, Ahmed Jalal
Shamsudin, A.A
Rahman, M.F
Nurzatul , N.Z
Rozihan, M
author_sort Khan Chowdhury, Ahmed Jalal
title Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
title_short Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
title_full Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
title_fullStr Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
title_full_unstemmed Growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (Isochrysis sp.) in laboratory cultured conditions
title_sort growth and total carotenoid, chlorophyll a and chlorophyll b of tropical microalgae (isochrysis sp.) in laboratory cultured conditions
publisher Asian Network for Scientific Information
publishDate 2013
url http://irep.iium.edu.my/34073/1/10-17.pdf
http://irep.iium.edu.my/34073/
http://scialert.net/jindex.php?issn=1727-3048
_version_ 1643610561376681984
score 13.222552

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