Cover Image

UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals

An ultra-performance liquid chromatography tandem mass spectrometry (UPLCeMS/MS) method is described for simultaneous determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1 and FB2), T-2 and HT-2 toxins in cereals. Mycoto...

Full description

Saved in:
Bibliographic Details
Main Authors: Soleimany, F., Jinap, Selamat, Abas, Faridah, Khatib, Alfi
Format: Article
Language:English
Published: Elsevier 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/33502/1/Soleimany_et_al.%2C_2012.pdf
http://irep.iium.edu.my/33502/
http://www.sciencedirect.com/science/article/pii/S0956713511004944
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.33502
record_format dspace
spelling my.iium.irep.335022013-12-18T02:40:43Z http://irep.iium.edu.my/33502/ UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals Soleimany, F. Jinap, Selamat Abas, Faridah Khatib, Alfi QD Chemistry An ultra-performance liquid chromatography tandem mass spectrometry (UPLCeMS/MS) method is described for simultaneous determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1 and FB2), T-2 and HT-2 toxins in cereals. Mycotoxins were separated by reverse phase liquid chromatography (RP-LC) and detected by tandem mass spectrometry using an electro spray-ionization interface (ESI) in both positive- and negative- ion modes. The mean recoveries of mycotoxins from spiked cereals ranged from 83.5% to 107.3%, whereas the limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 25 ng/g and 0.02e40 ng/g, respectively. The multi-mycotoxin method developed in this work was applied for the simultaneous determination of mycotoxins in 80 cereal samples collected from Malaysian markets. A total of 60 cereal samples (75%) were contaminated with at least one of these mycotoxins at levels greater than the LOD. Only one maize sample and two rice samples were contaminated at levels exceeding the European regulatory limits for aflatoxins and OTA (4 and 5 ng/g, respectively). The rates of the occurrence of mycotoxins in the commercial cereal samples were 50, 30, 19, 30, 16, 14, 14 and 12% for the aflatoxins (the total amount of AFB1, AFB2, AFG1 and AFG2), OTA, ZEA, DON, FB1, FB2, T-2 and HT-2 toxins, respectively. The results demonstrated that the procedure was suitable for the simultaneous determination of these mycotoxins in cereals and could be performed for their routine analysis in mycotoxin laboratories. Elsevier 2012-03-06 Article REM application/pdf en http://irep.iium.edu.my/33502/1/Soleimany_et_al.%2C_2012.pdf Soleimany, F. and Jinap, Selamat and Abas, Faridah and Khatib, Alfi (2012) UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals. Food Control, 25 (2). pp. 647-653. ISSN 0956-7135 http://www.sciencedirect.com/science/article/pii/S0956713511004944 10.1016/j.foodcont.2011.11.012
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Soleimany, F.
Jinap, Selamat
Abas, Faridah
Khatib, Alfi
UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
description An ultra-performance liquid chromatography tandem mass spectrometry (UPLCeMS/MS) method is described for simultaneous determination of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1 and FB2), T-2 and HT-2 toxins in cereals. Mycotoxins were separated by reverse phase liquid chromatography (RP-LC) and detected by tandem mass spectrometry using an electro spray-ionization interface (ESI) in both positive- and negative- ion modes. The mean recoveries of mycotoxins from spiked cereals ranged from 83.5% to 107.3%, whereas the limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 25 ng/g and 0.02e40 ng/g, respectively. The multi-mycotoxin method developed in this work was applied for the simultaneous determination of mycotoxins in 80 cereal samples collected from Malaysian markets. A total of 60 cereal samples (75%) were contaminated with at least one of these mycotoxins at levels greater than the LOD. Only one maize sample and two rice samples were contaminated at levels exceeding the European regulatory limits for aflatoxins and OTA (4 and 5 ng/g, respectively). The rates of the occurrence of mycotoxins in the commercial cereal samples were 50, 30, 19, 30, 16, 14, 14 and 12% for the aflatoxins (the total amount of AFB1, AFB2, AFG1 and AFG2), OTA, ZEA, DON, FB1, FB2, T-2 and HT-2 toxins, respectively. The results demonstrated that the procedure was suitable for the simultaneous determination of these mycotoxins in cereals and could be performed for their routine analysis in mycotoxin laboratories.
format Article
author Soleimany, F.
Jinap, Selamat
Abas, Faridah
Khatib, Alfi
author_facet Soleimany, F.
Jinap, Selamat
Abas, Faridah
Khatib, Alfi
author_sort Soleimany, F.
title UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
title_short UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
title_full UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
title_fullStr UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
title_full_unstemmed UPLCeMS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins, T-2 toxin and HT-2 toxin, in cereals
title_sort uplcems/ms for simultaneous determination of aflatoxins, ochratoxin a, zearalenone, don, fumonisins, t-2 toxin and ht-2 toxin, in cereals
publisher Elsevier
publishDate 2012
url http://irep.iium.edu.my/33502/1/Soleimany_et_al.%2C_2012.pdf
http://irep.iium.edu.my/33502/
http://www.sciencedirect.com/science/article/pii/S0956713511004944
_version_ 1643610452464238592
score 13.211869

Similar Items