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Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system

A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column d...

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Main Authors: Soleimany, F., Jinap, Selamat, Rahmani, Anoshep, Khatib, Alfi
Format: Article
Language:English
Published: Taylor and Francis 2011
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf
http://irep.iium.edu.my/33304/
http://www.tandfonline.com/doi/full/10.1080/19440049.2010.551547#.Uqp_DeKuqKI
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spelling my.iium.irep.333042013-12-13T03:24:57Z http://irep.iium.edu.my/33304/ Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system Soleimany, F. Jinap, Selamat Rahmani, Anoshep Khatib, Alfi QD Chemistry A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72–111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals. Taylor and Francis 2011-04-04 Article REM application/pdf en http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf Soleimany, F. and Jinap, Selamat and Rahmani, Anoshep and Khatib, Alfi (2011) Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. Food Additives & Contaminants: Part A, 28 (4). pp. 494-501. ISSN 1944-0049 http://www.tandfonline.com/doi/full/10.1080/19440049.2010.551547#.Uqp_DeKuqKI 10.1080/19440049.2010.551547
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Soleimany, F.
Jinap, Selamat
Rahmani, Anoshep
Khatib, Alfi
Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
description A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72–111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals.
format Article
author Soleimany, F.
Jinap, Selamat
Rahmani, Anoshep
Khatib, Alfi
author_facet Soleimany, F.
Jinap, Selamat
Rahmani, Anoshep
Khatib, Alfi
author_sort Soleimany, F.
title Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
title_short Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
title_full Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
title_fullStr Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
title_full_unstemmed Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
title_sort simultaneous detection of 12 mycotoxins in cereals using rp-hplc-pda-fld with phred and a post-column derivatization system
publisher Taylor and Francis
publishDate 2011
url http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf
http://irep.iium.edu.my/33304/
http://www.tandfonline.com/doi/full/10.1080/19440049.2010.551547#.Uqp_DeKuqKI
_version_ 1643610410752933888
score 13.211869

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