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Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Metho...

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Main Authors: Mamat, Siti Syariah, Kamarolzaman, Mohammad Fauzi Fahmi, Yahya, Farhana, Mahmood, Nur Diyana, Shahril, Mohamad Syahmi, Jakius, Krystal Fredolin, Mohtaruddin, Norhafizah, Darnis, Deny Susanti, Bakhtiar, M. Taher, Zakaria, Zainul Amiruddin
Format: Article
Language:English
Published: BioMed Central 2013
Subjects:
RS Pharmacy and materia medica
Online Access:http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf
http://irep.iium.edu.my/33006/
http://www.biomedcentral.com/1472-6882/13/326/abstract
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spelling my.iium.irep.330062014-12-08T09:38:18Z http://irep.iium.edu.my/33006/ Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats Mamat, Siti Syariah Kamarolzaman, Mohammad Fauzi Fahmi Yahya, Farhana Mahmood, Nur Diyana Shahril, Mohamad Syahmi Jakius, Krystal Fredolin Mohtaruddin, Norhafizah Darnis, Deny Susanti Bakhtiar, M. Taher Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Methods The respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay). Results MEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents. Conclusions The MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids. BioMed Central 2013-11-23 Article REM application/pdf en http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf Mamat, Siti Syariah and Kamarolzaman, Mohammad Fauzi Fahmi and Yahya, Farhana and Mahmood, Nur Diyana and Shahril, Mohamad Syahmi and Jakius, Krystal Fredolin and Mohtaruddin, Norhafizah and Darnis, Deny Susanti and Bakhtiar, M. Taher and Zakaria, Zainul Amiruddin (2013) Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats. BMC Complementary and Alternative Medicine, 13. p. 326. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/13/326/abstract 10.1186/1472-6882-13-326
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Mamat, Siti Syariah
Kamarolzaman, Mohammad Fauzi Fahmi
Yahya, Farhana
Mahmood, Nur Diyana
Shahril, Mohamad Syahmi
Jakius, Krystal Fredolin
Mohtaruddin, Norhafizah
Darnis, Deny Susanti
Bakhtiar, M. Taher
Zakaria, Zainul Amiruddin
Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
description Background Melastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model. Methods The respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay). Results MEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents. Conclusions The MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids.
format Article
author Mamat, Siti Syariah
Kamarolzaman, Mohammad Fauzi Fahmi
Yahya, Farhana
Mahmood, Nur Diyana
Shahril, Mohamad Syahmi
Jakius, Krystal Fredolin
Mohtaruddin, Norhafizah
Darnis, Deny Susanti
Bakhtiar, M. Taher
Zakaria, Zainul Amiruddin
author_facet Mamat, Siti Syariah
Kamarolzaman, Mohammad Fauzi Fahmi
Yahya, Farhana
Mahmood, Nur Diyana
Shahril, Mohamad Syahmi
Jakius, Krystal Fredolin
Mohtaruddin, Norhafizah
Darnis, Deny Susanti
Bakhtiar, M. Taher
Zakaria, Zainul Amiruddin
author_sort Mamat, Siti Syariah
title Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_short Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_full Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_fullStr Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_full_unstemmed Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
title_sort methanol extract of melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats
publisher BioMed Central
publishDate 2013
url http://irep.iium.edu.my/33006/1/BMC_Health_Melastoma.pdf
http://irep.iium.edu.my/33006/
http://www.biomedcentral.com/1472-6882/13/326/abstract
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score 13.211869

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