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In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile

This study was performed to evaluate the antifungal activities of the methanolic fractions from the stem bark of Entada spiralis Ridl. against human dermatophytes and yeast-like fungus in in vitro. Three types of human dermatophytes and one yeast-like fungus namely Trichophyton mentagrophytes ATCC...

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Main Authors: Harun, Aiza, Mat So'ad, Siti Zaiton, Mohd Hassan, Norazian, Che Mohd Ramli, Neni Kartini
Format: Article
Language:English
English
Published: Universiti Putra Malaysia Press 2014
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/32628/1/32628.pdf
http://irep.iium.edu.my/32628/4/32628_In-vitro%20antidermatophytic%20activity.SCOPUSpdf.pdf
http://irep.iium.edu.my/32628/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2022%20(1)%20Jan.%202014/11%20Page%20113-122%20(JST%200313-2011).pdf
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spelling my.iium.irep.326282017-09-19T02:18:16Z http://irep.iium.edu.my/32628/ In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile Harun, Aiza Mat So'ad, Siti Zaiton Mohd Hassan, Norazian Che Mohd Ramli, Neni Kartini QD Chemistry This study was performed to evaluate the antifungal activities of the methanolic fractions from the stem bark of Entada spiralis Ridl. against human dermatophytes and yeast-like fungus in in vitro. Three types of human dermatophytes and one yeast-like fungus namely Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102, Trichophyton tonsurans ATCC 28942, and Candida glabrata ATCC 66032 were tested against the methanolic fractions labelled as FA1, FA4 and FA5. First, the activities of antifungal were studied by disc diffusion method and broth micro dilution method. Second, we used an agar overlay bioautography assay to reveal antidermatophytic compounds of methanolic fractions. T.mentagrophytes, T.tonsuran and M.gypseum were susceptible towards all tested fractions in a concentration-dependent manner whereas C.glabrata was resistant. Fraction FA1 at a concentration of 400 mg/mL was found to exhibit highest antifungal activity with inhibition zone diameter of 22mm (T.mentagrophytes). FA1 showed MIC at 0.097 mg/mL while the MIC value for FA4 and FA5 were 3.12 mg/ml and 1.56 mg/ml respectively. Nystatin was used as standard antifungal drug and served as positive control. A garoverlay bioautography assay results showed that most of the bioactive compounds were 3 found in FA1. Based on these findings, it can be concluded that the stem bark extracts of E. spiralis can be a future source of potent natural antimicrobial drugs for superficial skin diseases. Universiti Putra Malaysia Press 2014 Article REM application/pdf en http://irep.iium.edu.my/32628/1/32628.pdf application/pdf en http://irep.iium.edu.my/32628/4/32628_In-vitro%20antidermatophytic%20activity.SCOPUSpdf.pdf Harun, Aiza and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian and Che Mohd Ramli, Neni Kartini (2014) In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile. Pertanika Journal of Science and Technology, 22 (1). pp. 113-121. ISSN 2231-8526 (O) 0128-7680 (P) http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2022%20(1)%20Jan.%202014/11%20Page%20113-122%20(JST%200313-2011).pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Harun, Aiza
Mat So'ad, Siti Zaiton
Mohd Hassan, Norazian
Che Mohd Ramli, Neni Kartini
In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
description This study was performed to evaluate the antifungal activities of the methanolic fractions from the stem bark of Entada spiralis Ridl. against human dermatophytes and yeast-like fungus in in vitro. Three types of human dermatophytes and one yeast-like fungus namely Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102, Trichophyton tonsurans ATCC 28942, and Candida glabrata ATCC 66032 were tested against the methanolic fractions labelled as FA1, FA4 and FA5. First, the activities of antifungal were studied by disc diffusion method and broth micro dilution method. Second, we used an agar overlay bioautography assay to reveal antidermatophytic compounds of methanolic fractions. T.mentagrophytes, T.tonsuran and M.gypseum were susceptible towards all tested fractions in a concentration-dependent manner whereas C.glabrata was resistant. Fraction FA1 at a concentration of 400 mg/mL was found to exhibit highest antifungal activity with inhibition zone diameter of 22mm (T.mentagrophytes). FA1 showed MIC at 0.097 mg/mL while the MIC value for FA4 and FA5 were 3.12 mg/ml and 1.56 mg/ml respectively. Nystatin was used as standard antifungal drug and served as positive control. A garoverlay bioautography assay results showed that most of the bioactive compounds were 3 found in FA1. Based on these findings, it can be concluded that the stem bark extracts of E. spiralis can be a future source of potent natural antimicrobial drugs for superficial skin diseases.
format Article
author Harun, Aiza
Mat So'ad, Siti Zaiton
Mohd Hassan, Norazian
Che Mohd Ramli, Neni Kartini
author_facet Harun, Aiza
Mat So'ad, Siti Zaiton
Mohd Hassan, Norazian
Che Mohd Ramli, Neni Kartini
author_sort Harun, Aiza
title In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
title_short In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
title_full In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
title_fullStr In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
title_full_unstemmed In-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
title_sort in-vitro antidermatophytic activity of methanolic fraction from entada spiralis ridl. stem bark and its bioautographic profile
publisher Universiti Putra Malaysia Press
publishDate 2014
url http://irep.iium.edu.my/32628/1/32628.pdf
http://irep.iium.edu.my/32628/4/32628_In-vitro%20antidermatophytic%20activity.SCOPUSpdf.pdf
http://irep.iium.edu.my/32628/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2022%20(1)%20Jan.%202014/11%20Page%20113-122%20(JST%200313-2011).pdf
_version_ 1643610263343071232
score 13.211869

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