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Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma

A simple liquid chromatographic method for the determination of gemifloxacin (CAS number 175463-14-6) in human plasma has been developed. An aliquot quantity of 1 mL plasma sample was taken and 0.1 mL internal standard was added and mixed. 1 mL methanol was added to it. The mixture was then sonicate...

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Main Authors: Chakrabarty, Uday Sankar, Das, Ayan, Bhaumik, Uttam, Chatterjee, Bappaditya, Nandi, Utpal, Pal, Tapan Kumar
Format: Article
Language:English
Published: Vieweg Verlag 2009
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/31911/1/Paper7.pdf
http://irep.iium.edu.my/31911/
http://link.springer.com/article/10.1365%2Fs10337-009-0981-7
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spelling my.iium.irep.319112013-09-20T04:15:10Z http://irep.iium.edu.my/31911/ Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma Chakrabarty, Uday Sankar Das, Ayan Bhaumik, Uttam Chatterjee, Bappaditya Nandi, Utpal Pal, Tapan Kumar R Medicine (General) A simple liquid chromatographic method for the determination of gemifloxacin (CAS number 175463-14-6) in human plasma has been developed. An aliquot quantity of 1 mL plasma sample was taken and 0.1 mL internal standard was added and mixed. 1 mL methanol was added to it. The mixture was then sonicated for 10 min followed by 20 min centrifugation at 5000 rpm (g = 3600). The supernatant layer was separated and filtered through simple filtration unit (membrane filter, 0.45 μm) and injected into the LC system consisting of Hypersil BDS, C18 (250 × 4.6 mm, 5 μm particle size) column, using 1% formic acid : methanol = 65:35 (v/v) as mobile phase with ultra violet detection at 328 nm. Lower limit of detection was 20 ng mL−1 and lower limit of quantitation was 50 ng mL−1. Maximum between-run precision was 14.614%. Mean extraction recovery was found to be 87.32 to 89.32%. Stability study showed that after three freeze-thaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 12 h and at −20 °C for 3 months. Before injecting into LC system, the processed samples were stable for at least 8 h. The method was used to perform bioequivalence study in human volunteers. Vieweg Verlag 2009-05-01 Article REM application/pdf en http://irep.iium.edu.my/31911/1/Paper7.pdf Chakrabarty, Uday Sankar and Das, Ayan and Bhaumik, Uttam and Chatterjee, Bappaditya and Nandi, Utpal and Pal, Tapan Kumar (2009) Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma. Chromatographia, 69 (9-10). pp. 853-858. ISSN 1612-1112 (O), 0009-5893 (P) http://link.springer.com/article/10.1365%2Fs10337-009-0981-7 10.1365/s10337-009-0981-7
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Chakrabarty, Uday Sankar
Das, Ayan
Bhaumik, Uttam
Chatterjee, Bappaditya
Nandi, Utpal
Pal, Tapan Kumar
Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
description A simple liquid chromatographic method for the determination of gemifloxacin (CAS number 175463-14-6) in human plasma has been developed. An aliquot quantity of 1 mL plasma sample was taken and 0.1 mL internal standard was added and mixed. 1 mL methanol was added to it. The mixture was then sonicated for 10 min followed by 20 min centrifugation at 5000 rpm (g = 3600). The supernatant layer was separated and filtered through simple filtration unit (membrane filter, 0.45 μm) and injected into the LC system consisting of Hypersil BDS, C18 (250 × 4.6 mm, 5 μm particle size) column, using 1% formic acid : methanol = 65:35 (v/v) as mobile phase with ultra violet detection at 328 nm. Lower limit of detection was 20 ng mL−1 and lower limit of quantitation was 50 ng mL−1. Maximum between-run precision was 14.614%. Mean extraction recovery was found to be 87.32 to 89.32%. Stability study showed that after three freeze-thaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 12 h and at −20 °C for 3 months. Before injecting into LC system, the processed samples were stable for at least 8 h. The method was used to perform bioequivalence study in human volunteers.
format Article
author Chakrabarty, Uday Sankar
Das, Ayan
Bhaumik, Uttam
Chatterjee, Bappaditya
Nandi, Utpal
Pal, Tapan Kumar
author_facet Chakrabarty, Uday Sankar
Das, Ayan
Bhaumik, Uttam
Chatterjee, Bappaditya
Nandi, Utpal
Pal, Tapan Kumar
author_sort Chakrabarty, Uday Sankar
title Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
title_short Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
title_full Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
title_fullStr Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
title_full_unstemmed Rapid and sensitive LC method for the analysis of Gemifloxacin in Human plasma
title_sort rapid and sensitive lc method for the analysis of gemifloxacin in human plasma
publisher Vieweg Verlag
publishDate 2009
url http://irep.iium.edu.my/31911/1/Paper7.pdf
http://irep.iium.edu.my/31911/
http://link.springer.com/article/10.1365%2Fs10337-009-0981-7
_version_ 1643610083044622336
score 13.211869

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