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Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice

Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migra...

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Main Authors: Abdalrahim , FA Aisha, Zhari I, Ismail, Khalid , M Abu-Saleh, Siddiqui, Mohammad Jamshed Ahmad, Ghafar, Gheniya, Shah, Amin Malik
Format: Article
Language:English
Published: BioMed Central 2013
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf
http://irep.iium.edu.my/30791/
http://www.biomedcentral.com/1472-6882/13/168
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spelling my.iium.irep.307912013-08-03T07:08:05Z http://irep.iium.edu.my/30791/ Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik QD Chemistry Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid. BioMed Central 2013-07-12 Article REM application/pdf en http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf Abdalrahim , FA Aisha and Zhari I, Ismail and Khalid , M Abu-Saleh and Siddiqui, Mohammad Jamshed Ahmad and Ghafar, Gheniya and Shah, Amin Malik (2013) Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice. BMC Complementary and Alternative Medicine (13). p. 168. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/13/168
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Abdalrahim , FA Aisha
Zhari I, Ismail
Khalid , M Abu-Saleh
Siddiqui, Mohammad Jamshed Ahmad
Ghafar, Gheniya
Shah, Amin Malik
Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
description Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid.
format Article
author Abdalrahim , FA Aisha
Zhari I, Ismail
Khalid , M Abu-Saleh
Siddiqui, Mohammad Jamshed Ahmad
Ghafar, Gheniya
Shah, Amin Malik
author_facet Abdalrahim , FA Aisha
Zhari I, Ismail
Khalid , M Abu-Saleh
Siddiqui, Mohammad Jamshed Ahmad
Ghafar, Gheniya
Shah, Amin Malik
author_sort Abdalrahim , FA Aisha
title Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_short Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_full Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_fullStr Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_full_unstemmed Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
title_sort syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
publisher BioMed Central
publishDate 2013
url http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf
http://irep.iium.edu.my/30791/
http://www.biomedcentral.com/1472-6882/13/168
_version_ 1643609938102059008
score 13.211869

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