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Metallothionein isogene transcription in red blood cell precursors from human cord blood

The in vitro transcription patterns for 10 functional metallothionein (MT) isogenes have been investigated in red blood cell (RBC) precursors from human cord blood. Active transcription status of the isogenes, MT-0, MT-1A, MT-1B, MT-1E, MT-1G, MT-1X, and MT-2A, was detected in both ex vivo expa...

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Main Authors: Rahman, Mohammad Tariqur, De Ley, Marc
Format: Article
Language:English
Published: Federation of European Biochemical Societies 2001
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/29704/1/Eur_J_Biochem.pdf
http://irep.iium.edu.my/29704/
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1742-4658/issues
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spelling my.iium.irep.297042013-08-02T06:30:32Z http://irep.iium.edu.my/29704/ Metallothionein isogene transcription in red blood cell precursors from human cord blood Rahman, Mohammad Tariqur De Ley, Marc Q Science (General) The in vitro transcription patterns for 10 functional metallothionein (MT) isogenes have been investigated in red blood cell (RBC) precursors from human cord blood. Active transcription status of the isogenes, MT-0, MT-1A, MT-1B, MT-1E, MT-1G, MT-1X, and MT-2A, was detected in both ex vivo expanded RBC precursors (burst-forming unit-erythroid) and glycophorin A1 and CD711 cells separated by magnetic cell sorting. Transcription patterns of these isogenes were analyzed at different times of incubation with the addition of Zn supplement. In neither the ex vivo expanded precursors nor glycophorin A1 and CD711 cells could MT-1F and MT-3 be detected. Transcripts of MT-4 were detected in glycophorin A1 and CD711 cells. Erythropoietin-responsive constitutive transcriptionof MT-1X and possible interleukin-3-responsive downregulation of MT-2A in ex vivo expanded precursors reveal their effect on MT biosynthesis. Biosynthesis and induction of MT at the protein level in the RBC precursors was also demonstrated by immunoblotting. Keywords: erythropoietin; glycophorin A; interleukin-3; metallothionein; zinc status. Federation of European Biochemical Societies 2001 Article REM application/pdf en http://irep.iium.edu.my/29704/1/Eur_J_Biochem.pdf Rahman, Mohammad Tariqur and De Ley, Marc (2001) Metallothionein isogene transcription in red blood cell precursors from human cord blood. The FEBS, 268. pp. 849-856. ISSN 1742-4658 http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1742-4658/issues
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Rahman, Mohammad Tariqur
De Ley, Marc
Metallothionein isogene transcription in red blood cell precursors from human cord blood
description The in vitro transcription patterns for 10 functional metallothionein (MT) isogenes have been investigated in red blood cell (RBC) precursors from human cord blood. Active transcription status of the isogenes, MT-0, MT-1A, MT-1B, MT-1E, MT-1G, MT-1X, and MT-2A, was detected in both ex vivo expanded RBC precursors (burst-forming unit-erythroid) and glycophorin A1 and CD711 cells separated by magnetic cell sorting. Transcription patterns of these isogenes were analyzed at different times of incubation with the addition of Zn supplement. In neither the ex vivo expanded precursors nor glycophorin A1 and CD711 cells could MT-1F and MT-3 be detected. Transcripts of MT-4 were detected in glycophorin A1 and CD711 cells. Erythropoietin-responsive constitutive transcriptionof MT-1X and possible interleukin-3-responsive downregulation of MT-2A in ex vivo expanded precursors reveal their effect on MT biosynthesis. Biosynthesis and induction of MT at the protein level in the RBC precursors was also demonstrated by immunoblotting. Keywords: erythropoietin; glycophorin A; interleukin-3; metallothionein; zinc status.
format Article
author Rahman, Mohammad Tariqur
De Ley, Marc
author_facet Rahman, Mohammad Tariqur
De Ley, Marc
author_sort Rahman, Mohammad Tariqur
title Metallothionein isogene transcription in red blood cell precursors from human cord blood
title_short Metallothionein isogene transcription in red blood cell precursors from human cord blood
title_full Metallothionein isogene transcription in red blood cell precursors from human cord blood
title_fullStr Metallothionein isogene transcription in red blood cell precursors from human cord blood
title_full_unstemmed Metallothionein isogene transcription in red blood cell precursors from human cord blood
title_sort metallothionein isogene transcription in red blood cell precursors from human cord blood
publisher Federation of European Biochemical Societies
publishDate 2001
url http://irep.iium.edu.my/29704/1/Eur_J_Biochem.pdf
http://irep.iium.edu.my/29704/
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1742-4658/issues
_version_ 1643609741217234944
score 13.211869

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