Cover Image

Focused ion beam machining of cell culture dish

Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads...

Full description

Saved in:
Bibliographic Details
Main Author: Ali, Mohammad Yeakub
Format: Conference or Workshop Item
Language:English
Published: 2008
Subjects:
T Technology (General)
TJ Mechanical engineering and machinery
TS Manufactures
Online Access:http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf
http://irep.iium.edu.my/28914/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.28914
record_format dspace
spelling my.iium.irep.289142018-12-05T08:30:49Z http://irep.iium.edu.my/28914/ Focused ion beam machining of cell culture dish Ali, Mohammad Yeakub T Technology (General) TJ Mechanical engineering and machinery TS Manufactures Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy subculture of the cells without conventional trypsinization where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric nanopillars of few hundred nanometer of diameter. This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also machined using FIB which can be used as a master microtool for the mass production of polymer nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and 2 ?m respectively. 2008 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf Ali, Mohammad Yeakub (2008) Focused ion beam machining of cell culture dish. In: ISE Seminar 2008, 3rd March 2008, Thailand. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic T Technology (General)
TJ Mechanical engineering and machinery
TS Manufactures
spellingShingle T Technology (General)
TJ Mechanical engineering and machinery
TS Manufactures
Ali, Mohammad Yeakub
Focused ion beam machining of cell culture dish
description Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy subculture of the cells without conventional trypsinization where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric nanopillars of few hundred nanometer of diameter. This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also machined using FIB which can be used as a master microtool for the mass production of polymer nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and 2 ?m respectively.
format Conference or Workshop Item
author Ali, Mohammad Yeakub
author_facet Ali, Mohammad Yeakub
author_sort Ali, Mohammad Yeakub
title Focused ion beam machining of cell culture dish
title_short Focused ion beam machining of cell culture dish
title_full Focused ion beam machining of cell culture dish
title_fullStr Focused ion beam machining of cell culture dish
title_full_unstemmed Focused ion beam machining of cell culture dish
title_sort focused ion beam machining of cell culture dish
publishDate 2008
url http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf
http://irep.iium.edu.my/28914/
_version_ 1643617724598845440
score 13.211869

Similar Items