Cover Image

Immbolization of lipase on multi-walled carbon nanotubes

Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent...

Full description

Saved in:
Bibliographic Details
Main Authors: Alkhatib, Ma'an, Salleh, Hamzah, Sultan, Anans M.
Format: Conference or Workshop Item
Language:English
Published: 2008
Subjects:
TA401 Materials of engineering and construction
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf
http://irep.iium.edu.my/2613/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.2613
record_format dspace
spelling my.iium.irep.26132011-11-16T14:20:44Z http://irep.iium.edu.my/2613/ Immbolization of lipase on multi-walled carbon nanotubes Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. TA401 Materials of engineering and construction TP248.13 Biotechnology Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute. 2008 Conference or Workshop Item REM application/pdf en http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf Alkhatib, Ma'an and Salleh, Hamzah and Sultan, Anans M. (2008) Immbolization of lipase on multi-walled carbon nanotubes. In: 2008 International Conference on Environment (ICENV 2008), 15-17 December, 2008, G Hotel, Penang. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TA401 Materials of engineering and construction
TP248.13 Biotechnology
spellingShingle TA401 Materials of engineering and construction
TP248.13 Biotechnology
Alkhatib, Ma'an
Salleh, Hamzah
Sultan, Anans M.
Immbolization of lipase on multi-walled carbon nanotubes
description Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute.
format Conference or Workshop Item
author Alkhatib, Ma'an
Salleh, Hamzah
Sultan, Anans M.
author_facet Alkhatib, Ma'an
Salleh, Hamzah
Sultan, Anans M.
author_sort Alkhatib, Ma'an
title Immbolization of lipase on multi-walled carbon nanotubes
title_short Immbolization of lipase on multi-walled carbon nanotubes
title_full Immbolization of lipase on multi-walled carbon nanotubes
title_fullStr Immbolization of lipase on multi-walled carbon nanotubes
title_full_unstemmed Immbolization of lipase on multi-walled carbon nanotubes
title_sort immbolization of lipase on multi-walled carbon nanotubes
publishDate 2008
url http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf
http://irep.iium.edu.my/2613/
_version_ 1643604994362966016
score 13.211869

Similar Items