Method for virus propagation in a bioreactor
THE PRESENT INVENTION RETATES TO A METHOD FOR VIRUS PROPAGATION IN A BIOREACTOR (10) COMPRISING THE STEPS OF: FIRSTLY; CULTURING A CELL IN A BIOREACIORTO PRODUCE MORE CELLS TO FORM A BIOREACTOR CULTURE IN THE PRESENCE OF MTCROCARRTERS (11), WHERETN THE BTOREACTOR rS A SPTNNER VESSEL OR STIRRED TAN...
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| Main Authors: | , |
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| Format: | Patent |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/25811/1/virus_in_Bioreactor.pdf http://irep.iium.edu.my/25811/ |
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| Summary: | THE PRESENT INVENTION RETATES TO A METHOD FOR VIRUS PROPAGATION IN A BIOREACTOR (10) COMPRISING THE STEPS
OF: FIRSTLY; CULTURING A CELL IN A BIOREACIORTO PRODUCE MORE CELLS TO FORM A BIOREACTOR CULTURE IN THE
PRESENCE OF MTCROCARRTERS (11), WHERETN THE BTOREACTOR rS A SPTNNER VESSEL OR STIRRED TANK BTOREACTOR;
THEN, INOCULATING THE CETIS WTTH VIRUS (12); AND SUBSEQUENTLY, INCUBANNG THE VIRUS WITH THE BIOREACTOR
CULTURE (13), WITH TEMPEMTURE OF 37"C, AGTTATION SPEED OF 30-70 RPM, PH 7.2, AND DISSOLVED OXYGEN CONTENT
(PO2) OF 30olo; WHEREIN THE WRUS IS NEWCASTT"E DISEASE VIRUS (NDV) AND THE CELLS ARE VERO CELLS. |
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