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Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma

Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemi...

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Main Authors: Abdullah, Nor Zamzila, Ahmad Sufi, Nur Farhah, A.Talib, Norlelawati, Ku Zaifah, Norsidah, Muhammad, Siti Aeshah @ Naznin, Mohamed, Abdul Hadi
Format: Conference or Workshop Item
Language:English
Published: 2012
Subjects:
R Medicine (General)
Online Access:http://irep.iium.edu.my/24613/1/AbstracCPATH2012%282%29.pdf
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spelling my.iium.irep.246132022-04-07T01:59:57Z http://irep.iium.edu.my/24613/ Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma Abdullah, Nor Zamzila Ahmad Sufi, Nur Farhah A.Talib, Norlelawati Ku Zaifah, Norsidah Muhammad, Siti Aeshah @ Naznin Mohamed, Abdul Hadi R Medicine (General) Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemical markers for cardiovascular diseases. PON 1 activity is usually measured in serum (from plain container) while some other relevant cardiovascular parameters require anti-coagulated plasma. Collection of blood in many types of container may increase the cost and require a larger amount of blood to be collected. Apart from plasma in lithium heparin, the reliability of plasma in other anti-coagulated container such as potassium-citrate, potassium- oxalate/sodium-fluoride and CPDA (from blood collection bag) on PON 1 activity have not yet been clearly ascertained. The aim of this study was to compare and to study the correlation between the PON1 activity in serum and in plasma collected in various anti-coagulated containers. Methods: An experimental study was carried out on 50 volunteers. Blood samples were collected in plain container and containers with potassium-EDTA, lithium-heparin, potassium-citrate, potassium-oxalate/sodium-fluoride and CPDA. Serum and plasma were analyzed for PON1 activity spectrophotometrically after the hydrolysis of substrates paraoxon. Results: The PON1 activity in plasma from lithium-heparin container was slightly reduced but not statistically different (p=0.062) from that of serum (272.95U/L±85.11 vs 288.95±91). However, the PON1 activity was significantly reduced (p<0.001) to 224.13±74.78 U/L, 211.46±64.18 U/L, 184.32±60.06 U/L and 48.00±45.91 U/L respectively in plasma from potassium-citrate, CPDA, potassium-oxalate/sodium-flouride and potassium-EDTA container. There were significant positive correlations (p<0.001) between PON1 activity in serum with PON1 activity in all anti-coagulated plasma except for plasma in potassium-EDTA container (r=0.27, p=0.06). Conclusion: Our finding suggested that only serum and plasma in lithium-heparin container are suitable for the analysis of PON1 activity. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/24613/1/AbstracCPATH2012%282%29.pdf Abdullah, Nor Zamzila and Ahmad Sufi, Nur Farhah and A.Talib, Norlelawati and Ku Zaifah, Norsidah and Muhammad, Siti Aeshah @ Naznin and Mohamed, Abdul Hadi (2012) Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma. In: 11th Annual Scientifc Meeting, College of Pathologist, 8-10 June 2012, Crowne Plaza Mutiara, Kuala Lumpur.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Abdullah, Nor Zamzila
Ahmad Sufi, Nur Farhah
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Siti Aeshah @ Naznin
Mohamed, Abdul Hadi
Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
description Background: Paraoxonase 1 (PON1) is a high density lipoprotein (HDL) associated enzyme that is known for its function to hydrolyze organophosphate (OPs) into a relatively harmless substance and inhibit oxidative modification of low density lipoprotein (LDL). It is among the commonly studied biochemical markers for cardiovascular diseases. PON 1 activity is usually measured in serum (from plain container) while some other relevant cardiovascular parameters require anti-coagulated plasma. Collection of blood in many types of container may increase the cost and require a larger amount of blood to be collected. Apart from plasma in lithium heparin, the reliability of plasma in other anti-coagulated container such as potassium-citrate, potassium- oxalate/sodium-fluoride and CPDA (from blood collection bag) on PON 1 activity have not yet been clearly ascertained. The aim of this study was to compare and to study the correlation between the PON1 activity in serum and in plasma collected in various anti-coagulated containers. Methods: An experimental study was carried out on 50 volunteers. Blood samples were collected in plain container and containers with potassium-EDTA, lithium-heparin, potassium-citrate, potassium-oxalate/sodium-fluoride and CPDA. Serum and plasma were analyzed for PON1 activity spectrophotometrically after the hydrolysis of substrates paraoxon. Results: The PON1 activity in plasma from lithium-heparin container was slightly reduced but not statistically different (p=0.062) from that of serum (272.95U/L±85.11 vs 288.95±91). However, the PON1 activity was significantly reduced (p<0.001) to 224.13±74.78 U/L, 211.46±64.18 U/L, 184.32±60.06 U/L and 48.00±45.91 U/L respectively in plasma from potassium-citrate, CPDA, potassium-oxalate/sodium-flouride and potassium-EDTA container. There were significant positive correlations (p<0.001) between PON1 activity in serum with PON1 activity in all anti-coagulated plasma except for plasma in potassium-EDTA container (r=0.27, p=0.06). Conclusion: Our finding suggested that only serum and plasma in lithium-heparin container are suitable for the analysis of PON1 activity.
format Conference or Workshop Item
author Abdullah, Nor Zamzila
Ahmad Sufi, Nur Farhah
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Siti Aeshah @ Naznin
Mohamed, Abdul Hadi
author_facet Abdullah, Nor Zamzila
Ahmad Sufi, Nur Farhah
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Muhammad, Siti Aeshah @ Naznin
Mohamed, Abdul Hadi
author_sort Abdullah, Nor Zamzila
title Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_short Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_full Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_fullStr Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_full_unstemmed Paraoxonase-1 (PON1) activity in serum and various anticoagulated-plasma
title_sort paraoxonase-1 (pon1) activity in serum and various anticoagulated-plasma
publishDate 2012
url http://irep.iium.edu.my/24613/1/AbstracCPATH2012%282%29.pdf
http://irep.iium.edu.my/24613/
_version_ 1729702894652882944
score 13.211869

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