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Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma

Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive...

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Main Authors: Effendy Goon, Danial, Harun, Norra, Muhammad, Siti Aeshah @ Naznin, A.Talib, Norlelawati
Format: Conference or Workshop Item
Language:English
Published: 2012
Subjects:
RB Pathology
Online Access:http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf
http://irep.iium.edu.my/24612/
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spelling my.iium.irep.246122022-04-07T02:09:27Z http://irep.iium.edu.my/24612/ Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma Effendy Goon, Danial Harun, Norra Muhammad, Siti Aeshah @ Naznin A.Talib, Norlelawati RB Pathology Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf Effendy Goon, Danial and Harun, Norra and Muhammad, Siti Aeshah @ Naznin and A.Talib, Norlelawati (2012) Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma. In: 11th Annual Scientific Meeting, College of Pathologist Malaysia, 8-10 June 2012, Crowne Plaza Mutiara, Kuala Lumpur.
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RB Pathology
spellingShingle RB Pathology
Effendy Goon, Danial
Harun, Norra
Muhammad, Siti Aeshah @ Naznin
A.Talib, Norlelawati
Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
description Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma.
format Conference or Workshop Item
author Effendy Goon, Danial
Harun, Norra
Muhammad, Siti Aeshah @ Naznin
A.Talib, Norlelawati
author_facet Effendy Goon, Danial
Harun, Norra
Muhammad, Siti Aeshah @ Naznin
A.Talib, Norlelawati
author_sort Effendy Goon, Danial
title Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_short Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_full Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_fullStr Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_full_unstemmed Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_sort preliminary analyses on detection of syt-ssx fusion-transcripts in synovial sarcoma
publishDate 2012
url http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf
http://irep.iium.edu.my/24612/
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score 13.211869

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