Cover Image

Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens

Methods: In this study, proteomics methods have been used to study the effects of different currents and voltages used to stun chickens. Protein profiles of chicken hearts were constructed to detect differences in protein expression and modification. The different voltages studied were 10 V, 40 V...

Full description

Saved in:
Bibliographic Details
Main Authors: Amid, Azura, Abu Samah, Norshahida, Yusof, Faridah
Format: Article
Language:English
Published: BioMed Central Ltd 2012
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/23639/1/Identification_of_troponin_I.pdf
http://irep.iium.edu.my/23639/
http://www.proteomesci.com/content/10/1/1
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.iium.irep.23639
record_format dspace
spelling my.iium.irep.236392012-06-12T02:39:26Z http://irep.iium.edu.my/23639/ Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens Amid, Azura Abu Samah, Norshahida Yusof, Faridah Q Science (General) Methods: In this study, proteomics methods have been used to study the effects of different currents and voltages used to stun chickens. Protein profiles of chicken hearts were constructed to detect differences in protein expression and modification. The different voltages studied were 10 V, 40 V and 70 V, while the currents examined were 0.25 A, 0.5 A, and 0.75 A. The profiles obtained from these stunning conditions were compared to the nonstunned (0 A, 0 V) sample. Results: Proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identified troponin I and alpha cardiac muscle actin 1 in the electrically stimulated heart samples. The overexpression of the proteins was further confirmed at the transcriptional level by Real Time PCR. Conclusion: The results from MALDI-TOF and Real Time PCR agreed; therefore, this method for identifying biomarkers of electrically stimulated chicken hearts provides a novel approach for differentiation the hearts of increased electrically stimulated chickens from those of non-stunned chickens. BioMed Central Ltd 2012-01-10 Article REM application/pdf en http://irep.iium.edu.my/23639/1/Identification_of_troponin_I.pdf Amid, Azura and Abu Samah, Norshahida and Yusof, Faridah (2012) Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens. Proteome Science, 10 (1). pp. 1-8. ISSN 1477-5956 http://www.proteomesci.com/content/10/1/1 10.1186/1477-5956-10-1
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Amid, Azura
Abu Samah, Norshahida
Yusof, Faridah
Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
description Methods: In this study, proteomics methods have been used to study the effects of different currents and voltages used to stun chickens. Protein profiles of chicken hearts were constructed to detect differences in protein expression and modification. The different voltages studied were 10 V, 40 V and 70 V, while the currents examined were 0.25 A, 0.5 A, and 0.75 A. The profiles obtained from these stunning conditions were compared to the nonstunned (0 A, 0 V) sample. Results: Proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry (MALDI-TOF) identified troponin I and alpha cardiac muscle actin 1 in the electrically stimulated heart samples. The overexpression of the proteins was further confirmed at the transcriptional level by Real Time PCR. Conclusion: The results from MALDI-TOF and Real Time PCR agreed; therefore, this method for identifying biomarkers of electrically stimulated chicken hearts provides a novel approach for differentiation the hearts of increased electrically stimulated chickens from those of non-stunned chickens.
format Article
author Amid, Azura
Abu Samah, Norshahida
Yusof, Faridah
author_facet Amid, Azura
Abu Samah, Norshahida
Yusof, Faridah
author_sort Amid, Azura
title Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
title_short Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
title_full Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
title_fullStr Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
title_full_unstemmed Identification of troponin I and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
title_sort identification of troponin i and actin, alpha cardiac muscle 1 as potential biomarkers for hearts of electrically stimulated chickens
publisher BioMed Central Ltd
publishDate 2012
url http://irep.iium.edu.my/23639/1/Identification_of_troponin_I.pdf
http://irep.iium.edu.my/23639/
http://www.proteomesci.com/content/10/1/1
_version_ 1643608620738281472
score 13.211869

Similar Items