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Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system

An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate com...

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Main Authors: Mehrnoush, Amid, Sarker, Md. Zaidul Islam, Mustafa, Shuhaimi, Mohd Yazid, Abdul Manap
Format: Article
Language:English
Published: Molecular Diversity Preservation International (MDPI) 2011
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf
http://irep.iium.edu.my/17926/
http://www.mdpi.com/1420-3049/16/10/8419/
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spelling my.iium.irep.179262012-02-21T03:16:20Z http://irep.iium.edu.my/17926/ Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system Mehrnoush, Amid Sarker, Md. Zaidul Islam Mustafa, Shuhaimi Mohd Yazid, Abdul Manap QD Chemistry An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate composition (12–20%, w/w),system pH (6–9), and addition of different concentrations of neutral salts (0–8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel. Molecular Diversity Preservation International (MDPI) 2011-10-10 Article REM application/pdf en http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf Mehrnoush, Amid and Sarker, Md. Zaidul Islam and Mustafa, Shuhaimi and Mohd Yazid, Abdul Manap (2011) Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system. Molecules , 16 (10). pp. 8419-8427. ISSN 1420-3049 http://www.mdpi.com/1420-3049/16/10/8419/ DOI:10.3390/molecules16108419
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Mehrnoush, Amid
Sarker, Md. Zaidul Islam
Mustafa, Shuhaimi
Mohd Yazid, Abdul Manap
Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
description An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate composition (12–20%, w/w),system pH (6–9), and addition of different concentrations of neutral salts (0–8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel.
format Article
author Mehrnoush, Amid
Sarker, Md. Zaidul Islam
Mustafa, Shuhaimi
Mohd Yazid, Abdul Manap
author_facet Mehrnoush, Amid
Sarker, Md. Zaidul Islam
Mustafa, Shuhaimi
Mohd Yazid, Abdul Manap
author_sort Mehrnoush, Amid
title Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
title_short Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
title_full Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
title_fullStr Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
title_full_unstemmed Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
title_sort direct purification of pectinase from mango (mangifera indica cv. chokanan) peel using a peg/salt-based aqueous two phase system
publisher Molecular Diversity Preservation International (MDPI)
publishDate 2011
url http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf
http://irep.iium.edu.my/17926/
http://www.mdpi.com/1420-3049/16/10/8419/
_version_ 1643607380193181696
score 13.211869

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